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Initial development of a multistage cancer model based on Syrian hamster embryo (SHE) cell transformation studies

dc.contributor.authorLiao, Kai-Hsin, author
dc.contributor.authorYang, Raymond Shih-hsien, 1940-, advisor
dc.contributor.authorReardon, Kenneth F., advisor
dc.contributor.authorMurphy, V. A., committee member
dc.descriptionDepartment Head: V. A. Murphy.
dc.description.abstractTo better incorporate biologic information into quantitative cancer modeling, the two-stage MVK (Moolgavkar-Venzon-Knudson) model has been modified for use with SHE cell neoplastic progression. Conceptually, five phenotypic stages are included in this model: normal cells can either become senescent or mutate into immortal cells followed by anchorage-independent growth and tumorigenic stages. Cells in each stage have distinct division, death and mutation rates, and mutation is assumed to occur during cell division. Model development and related experiments were focused on studying the abilities of lead, arsenic, chromium, and a mixture of these three metals to induce progression of SHE cells from one phenotype to the next. Cell division and death rates were assessed using flow cytometric analysis for inclusion in the model. Cell division rates were measured using bromodeoxyuridine (BrdU) incorporation with propidium iodide staining, which allows for the calculation of potential doubling time, a measure of cell cycle time that takes growth fraction, but not cell loss, into account. Potential doubling times of normal SHE cells ranged from 12 to 59 hours, depending on the degree of confluence of cell cultures. Cell death was measured by a flow cytometry method based on propidium iodide staining specifically related to membrane damage. The mean cell death rate is approximately equal to 1 % of the average value of division rates. The individual metals and their mixture did not induce immortalization or further mutations of SHE cells in our laboratory following a 2-day exposure. However, the growth of SHE cells was inhibited by 5.4 μM of arsenic, with cells becoming senescent after only 16 population doublings; whereas, normal cells and cells exposed to lower arsenic concentrations lasted for at least 30 population doublings. The model developed in our laboratory successfully predicted the growth of normal cells. The cell senescence rates under the impact of arsenic exposure were also calculated. Mechanisms responsible for induction of cellular senescence in SHE cells exposed to arsenic may be involved in the apparent inability of arsenic to induce neoplasia in experimental animals.
dc.format.mediummasters theses
dc.publisherColorado State University. Libraries
dc.relationCatalog record number (MMS ID): 991007946199703361
dc.relationRC267.L47 1999
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see
dc.subjectcancer modeling
dc.subjectSHE cells
dc.subjectMoolgavkar-Venzon-Knudson model
dc.subjectSyrian hamster embryo cells
dc.subjectbiologically-based dose-response model
dc.subjectBBDR model
dc.subjectMVK model
dc.subject.lcshCancer -- Research
dc.subject.lcshCancer -- Animal models
dc.titleInitial development of a multistage cancer model based on Syrian hamster embryo (SHE) cell transformation studies
dcterms.rights.dplaThis Item is protected by copyright and/or related rights ( You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). and Bioresource Engineering State University of Science (M.S.)


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