Alkaline/peracetic acid as a pretreatment of lignocellulosic biomass for ethanol fuel production
| dc.contributor.author | Teixeira, Lincoln Cambraia, author | |
| dc.contributor.author | Crews, Donald L., advisor | |
| dc.contributor.author | Schroeder, Herbert A., committee member | |
| dc.contributor.author | Linden, James C., committee member | |
| dc.contributor.author | Wangaard, Frederick F., committee member | |
| dc.date.accessioned | 2022-02-04T20:22:49Z | |
| dc.date.available | 2022-02-04T20:22:49Z | |
| dc.date.issued | 1998 | |
| dc.description.abstract | Peracetic add is a lignin oxidation pretreatment with low energy input by which biomass can be treated in a silo type system for improving enzymatic digestibility of lignocellulosic materials for ethanol production. Experimentally, ground hybrid poplar wood and sugar cane bagasse are placed in plastic bags and a peracetic acid solution is added to the biomass in different concentrations based on oven-dry biomass. The ratio of solution to biomass is 6:1; after initial mixing of the resulting paste, a seven-day storage period at about 20 °C is used in this study. As a complementary method, a series of pre-pretreatments using stoichiometric amounts of sodium hydroxide and ammonium hydroxide based on 4-methyl-glucuronic acid and acetyl content in the biomass is been performed before addition of peracetic acid. The alkaline solutions are added to the biomass in a ratio of 14:1 solution to biomass: the slurry is mixed for 24 hours at ambient temperature. The above procedures give high xylan content substrates. Consequently, xylanase/beta- glucosidase combinations are more effective than cellulase preparations in hydrolyzing these materials. The pretreatment effectiveness is evaluated using standard enzymatic hydrolysis and simultaneous saccharification and cofermentation (SSCF) procedures. Hybrid poplar wood pretreated with 15 and 21% peracetic acid based on oven- dry weight of wood gives glucan conversion yields of 76.5 and 98.3%, respectively. Sugar cane bagasse pretreated with the same loadings gives corresponding yields of 85.9 and 93.1%. Raw wood and raw bagasse give corresponding yields of 6.8 and 28.8%, respectively. The combined 6% NaOH/15% peracetic acid pretreatments increase the glucan conversion yields from 76.5 to 100.0% for hybrid poplar wood and from 85.9 to 97.6% for sugar cane bagasse. Respective ethanol yields of 92.8 and 91.9% are obtained from 6% NaOH/15% peracetic acid pretreated materials using recombinant Zymomonas mobilis CP4/pZB5. Peracetic acid pretreatment improves enzymatic digestibility of hybrid poplar wood and sugar cane bagasse. Based on reduction of acetyl groups in the two lignocellulosic materials, alkaline pre-pretreatments are helpful in reducing peracetic acid requirements in the pretreatment and consequently diminishing growth inhibition of the bacteria that was observed using higher peracetic acid loadings. | |
| dc.format.medium | doctoral dissertations | |
| dc.identifier.uri | https://hdl.handle.net/10217/234366 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation | Catalog record number (MMS ID): 991005663269703361 | |
| dc.relation | TP593 .T45 1998 | |
| dc.relation.ispartof | 1980-1999 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject.lcsh | Wood-pulp | |
| dc.subject.lcsh | Lignocellulose | |
| dc.subject.lcsh | Alcohol | |
| dc.title | Alkaline/peracetic acid as a pretreatment of lignocellulosic biomass for ethanol fuel production | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Forest Sciences | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy (Ph.D) |
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