The surface proteome of Francisella tularensis
| dc.contributor.author | Chandler, Jeffrey Craig, author | |
| dc.contributor.author | Belisle, John T., advisor | |
| dc.contributor.author | Gilmore, Robert D., committee member | |
| dc.contributor.author | Goodridge, Lawrence D., committee member | |
| dc.contributor.author | Petersen, Jeannine M., committee member | |
| dc.date.accessioned | 2007-01-03T05:47:35Z | |
| dc.date.available | 2007-01-03T05:47:35Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | The surface associated lipids, polysaccharides, and proteins of bacterial pathogens often have significant roles in environmental and host-pathogen interactions. Lipopolysaccharide and an O-antigen polysaccharide capsule are the best defined Francisella tularensis surface molecules, and are important virulence factors that also contribute to the phenotypic variability of Francisella species, subspecies, and populations. In contrast, little is known regarding the composition and contributions of surface proteins in the biology of Francisella , or what roles they have in the documented phenotypic variability of this genus. A sufficient understanding of the Francisella surface proteome has been hampered by the few surface proteins identified and the inherent difficulty of characterizing new surface proteins. Thus, the objective of this dissertation was to provide an enhanced definition of F. tularensis surface proteome and evaluate how surface proteins relate to aspects of F. tularensis physiology, specifically humoral immunity and phenotypic variability of subspecies and populations. Analyses of the F. tularensis live vaccine strain surface proteome resulted in the identification of 36 proteins, 28 of which were newly described to the surface of this bacterium. Bioinformatic comparisons of surface proteins to their homologs in other Francisella species, subspecies, and populations revealed numerous differences that may contribute variable phenotypes, including significant alterations in the ChiA chitinase (FTL_1521). Given the utility of surface proteins in vaccines effective against other bacterial pathogens, the F. tularensis surface proteins recognized by three forms of vaccination were determined in a murine model. Immune serum derived from the most effective F. tularensis vaccine, F. tularensis live vaccine strain, recognized a small set of proteins, of which the majority of antigens were surface localized. In comparison, sera collected from mice vaccinated with two less effective subunit vaccines containing F. tularensis membrane and surface proteins recognized a much greater number of antigens. Although surface proteins were also recognized in response to subunit vaccinations, the majority of antigens were not surface associated. These data suggest that a targeted humoral response to a select set of identified surface proteins offers the greatest protective effect. Finally, the research presented in this dissertation provided the first biochemical characterization of the surface localized ChiA chitinase that was predicted to contribute to the phenotypic variability of Francisella biotypes. Multiple chitinases are often produced by a single organism which synergistically depolymerize chitin in nature. Thus, biochemical evaluations were extended to other F. tularensis and F. novicida chitinases, ChiB (FTT_1768), ChiC (FTW_0313), and ChiD (FTT_0066), that were identified by in silico analyses of Francisella genomes. Differences were noted between the chitinase genes and chitinase activities of Francisella species, subspecies, and populations. The chitinase activities observed for F. tularensis strains were predominantly associated with whole cell lysates, while the chitinase activities of F. novicida localized to the culture supernatant. The overall level of chitinase activity differed among the subpopulations of F. tularensis , and between F. tularensis and F. novicida . Recombinant production of the putative chitinases and enzymatic evaluations revealed ChiA, ChiB, ChiC, and ChiD possessed dissimilar chitinase activities. These biochemical studies coupled with bioinformatic analyses and the evaluation of chiA and chiC knockouts in F. tularensis A1 and A2 populations, respectively, provided a molecular basis to explain the differential chitinase activities observed among the species and subpopulations of Francisella . | |
| dc.format.medium | born digital | |
| dc.format.medium | doctoral dissertations | |
| dc.identifier | Chandler_colostate_0053A_10549.pdf | |
| dc.identifier.uri | http://hdl.handle.net/10217/50669 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2000-2019 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | surface antigens | |
| dc.subject | phenotypic variability | |
| dc.subject | surface proteins | |
| dc.subject.lcsh | Chitinase | |
| dc.subject.lcsh | Francisella | |
| dc.title | The surface proteome of Francisella tularensis | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Microbiology, Immunology, and Pathology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy (Ph.D.) |
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