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Effects of potential Tylosin substitutes on Salmonella prevalence and the microbiome of subiliac lymph nodes of beef feedlot cattle

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dc.contributor.authorHolzer, Katlyn, author
dc.contributor.authorMartin, Jennifer, advisor
dc.contributor.authorBelk, Keith, committee member
dc.contributor.authorMetcalf, Jessica, committee member
dc.contributor.authorSchountz, Tony, committee member
dc.date.accessioned2017-09-14T16:06:56Z
dc.date.available2017-09-14T16:06:56Z
dc.date.issued2017
dc.description.abstractTylosin, a macrolide antibiotic, is fed to feedlot cattle for liver abscess prevention. Tylosin alternatives are currently being investigated as pressures to reduce the amount of antibiotics used in livestock increase. Understanding effects of various feeding strategies on the safety of beef products is a priority as alternatives are investigated. This study investigated the effect of Tylosin, and two Tylosin alternatives on the prevalence of Salmonella in subiliac lymph nodes (SLN) and the microbiome of SLN from feedlot cattle. Salmonella harborage in the lymph node is a challenge for the beef industry as ground beef is made from beef trimmings that commonly contain lymph nodes. Consumption of contaminated ground beef is one of the possible foodborne routes of Salmonella infection. SLN (n=600) were collected from feedlot cattle (n = 5,481) at the time of slaughter. Overall 84.6% of the SLN were positive for Salmonella and the treatment did not influence prevalence (P > 0.8402). Samples from each pen of feedlot cattle (15 SLN/pen) were composited for microbiome analysis using 16s rRNA amplicon sequencing. Samples were analyzed using the open-source software Quantitative Insights Into Microbial Ecology (QIIME). The treatment did not influence the microbiome of the SLN (P = 0.223; P= 0.267). The top three phyla present were Proteobacteria (67.3%), Actinobacteria (10.2%), and Acidobacteria (9.5%). Although Salmonella was culturally isolated, it was not identified in the microbiome analysis because the genus could not be resolved for 18.9% of the Enterobacteriaceae family. Understanding why Salmonella is detectable in the lymph nodes may hold the key for prevention, and characterizing the microbiome is crucial for this process.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierHolzer_colostate_0053N_14421.pdf
dc.identifier.urihttps://hdl.handle.net/10217/184044
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectmicrobiome
dc.subjectTylosin
dc.subjectlymph node
dc.subjectSalmonella
dc.titleEffects of potential Tylosin substitutes on Salmonella prevalence and the microbiome of subiliac lymph nodes of beef feedlot cattle
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineAnimal Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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