Repository logo
 

Quantum dot studies with time-resolved super-resolution microscopy

Simple item page
dc.contributor.authorDunlap, Megan Kathryn, author
dc.contributor.authorVan Orden, Alan, advisor
dc.contributor.authorGelfand, Martin, advisor
dc.contributor.authorKrapf, Diego, committee member
dc.contributor.authorPrieto, Amy, committee member
dc.contributor.authorSzamel, Grzegorz, committee member
dc.date.accessioned2021-09-06T10:26:39Z
dc.date.available2021-09-06T10:26:39Z
dc.date.issued2021
dc.description.abstractQuantum dots (QDs) are semiconductor nanoparticles whose optical properties make them ideal candidates for a myriad of applications including fluorescence imaging and light harvesting technologies. They are highly emissive and their stochastic switching between states of low and high intensity, called blinking, lends them particularly well to super-resolution (SR) microscopy studies. This thesis is devoted to the development and application of a SR microscope with exceptionally high temporal resolution, so that the fluorescence lifetime, intensity, and emitter location can be simultaneously monitored. This time-resolved SR microscope is used to characterize CdSe/CdS core/shell QDs and clusters of QDs. Small clusters of ~2-5 QDs exhibited fluorescence intensities and lifetimes indicative of directed energy transfer, and regions were resolved within the clusters that were responsible for donating and accepting energy. Correlated images of the same clusters with scanning electron microscopy were used to verify the true distances between QDs in an attempt to confirm the distance-dependence of the Foerster energy transfer rate. A new analysis method was developed for resolving non-blinking emitters based on the lifetime information accessible with the time-resolved SR microscope.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierDunlap_colostate_0053A_16783.pdf
dc.identifier.urihttps://hdl.handle.net/10217/233860
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectlifetime
dc.subjectquantum dot
dc.subjectsuper-resolution
dc.subjectmaximum likelihood estimation
dc.subjectenergy transfer
dc.subjectsingle photon counting
dc.titleQuantum dot studies with time-resolved super-resolution microscopy
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dunlap_colostate_0053A_16783.pdf
Size:
6.04 MB
Format:
Adobe Portable Document Format
Download

Collections

2020-
Theses and Dissertations