Metabolic engineering interventions for sustainable 2,3-butanediol production in gas fermenting Clostridium autoethanogenum
| dc.contributor.author | Ghadermazi, Parsa, author | |
| dc.contributor.author | Chan, Siu Hung, advisor | |
| dc.contributor.author | Wrighton, Kelly, committee member | |
| dc.contributor.author | Reisfeld, Brad, committee member | |
| dc.date.accessioned | 2023-06-01T17:27:08Z | |
| dc.date.available | 2023-06-01T17:27:08Z | |
| dc.date.issued | 2023 | |
| dc.description | Zip file contains supplementary files 1 and 2. | |
| dc.description.abstract | Gas fermentation provides a promising platform to turn low-cost and readily available single-carbon waste gases into commodity chemicals such as 2,3-butanediol. Clostridium autoethanogenum is usually used as a robust and flexible chassis for gas fermentation. Here, we leveraged on constraints-based stoichiometric modeling and kinetic ensemble modeling of the C. autoethanogenum metabolic network to provide a systematic in silico analysis of metabolic engineering interventions for 2,3-butanediol overproduction and low carbon substrate loss in dissipated CO2. Our analysis allowed us to identify and to assess comparatively the expected performances for a wide range of single, double, and triple interventions. Our analysis managed to individuate bottleneck reactions in relevant metabolic pathways when suggesting intervening strategies. Besides recapitulating intuitive and/or previously attempted genetic modifications, our analysis neatly outlined that the interventions - at least partially - impinging on by-products branching from acetyl-CoA and pyruvate (acetate, ethanol, amino acids) offer valuable alternatives to the interventions focusing directly on the specific branch from pyruvate to 2,3-butanediol. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.format.medium | ZIP | |
| dc.format.medium | XLSX | |
| dc.identifier | Ghadermazi_colostate_0053N_17651.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/236580 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | metabolic engineering | |
| dc.subject | C. autoethanogenum | |
| dc.title | Metabolic engineering interventions for sustainable 2,3-butanediol production in gas fermenting Clostridium autoethanogenum | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Chemical and Biological Engineering | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |