Characterizing unique features of prion-like domains recruited to stress granules
| dc.contributor.author | Baer, Matthew Henry, author | |
| dc.contributor.author | Ross, Eric D., advisor | |
| dc.contributor.author | Stasevich, Timothy J., committee member | |
| dc.contributor.author | Zabel, Mark D., committee member | |
| dc.date.accessioned | 2022-01-07T11:29:17Z | |
| dc.date.available | 2024-01-06T11:29:17Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Upon nucleation into a cross-β-structure, a classical amyloid will elongate in a concentration-dependent manner as successive hydrogen bonds are made along each exposed β-strand. Solid-state assemblies in biomolecular condensates such as the Balbiani Body in oocytes share a similar β-sheet rich structure that contributes to low dynamicity of the interactions in this separated phase. Prion-like domains (PrLDs) are domains that compositionally resemble yeast prion domains. Some PrLDs can form solid-state assemblies; however, PrLDs have also been associated with the formation of more liquid-like biomolecular condensates. Liquid-like biomolecular condensates form upon varying stimuli, and they appear to assemble through different mechanisms of recruitment than what has been observed for solid-state assemblies. Many solid-state assemblies form via homotypic interactions between identical assembly-prone PrLDs. In contrast, liquid-like assemblies generally form through heterotypic interactions across multiple components. Both solid-state and liquid-like condensates recruit domains that are currently classified as "prion-like", but evidence is mounting that these contrasting condensates preferentially recruit functionally different types of prion-like domains. A unique feature of prion-like domains recruited to liquid-like condensates is that formation of these assemblies appears to obey a more diverse set of rules for recruitment. This diversity of recruitment of PrLDs may be largely due to the heterotypic interaction mechanism for assembly into liquid-like condensates. This mechanism of recruitment may also be partially responsible for the different compositional preferences observed for PrLDs recruited to liquid-like assemblies. PrLD manipulation is a convenient tool for analyzing the compositional elements contributing to formation of both classes of biomolecular condensates. To elucidate the divergent features of PrLDs recruited to liquid-like assemblies, we used systematic mutation of synthetic PrLDs, among other techniques, to examine their unique compositional requirements for assembly. We have created a set of synthetic PrLDs which demonstrates a gradient of assembly-propensity, where localization to stress granules is modulated by differences in residue composition of the domains. To measure the different degrees of assembly propensity of PrLDs, we developed a novel means of quantitative image analysis to determine the enrichment of PrLDs in stress granules. The goals of this work are to help uncover some of the compositional features unique to prion-like domains that are recruited to liquid-like assemblies such as stress granules, to introduce a novel method of image analysis that will allow for quantification of assembly propensity of PrLDs in yeast, and to probe the concentration dependence for assembly of PrLDs into stress granules. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier | Baer_colostate_0053N_16979.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/234209 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | prion-like domains | |
| dc.subject | LLPS | |
| dc.subject | stress granules | |
| dc.title | Characterizing unique features of prion-like domains recruited to stress granules | |
| dc.type | Text | |
| dcterms.embargo.expires | 2024-01-06 | |
| dcterms.embargo.terms | 2024-01-06 | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Biochemistry and Molecular Biology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
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