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Engineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells

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dc.contributor.authorDePorter, Sandra M., author
dc.contributor.authorMcNaughton, Brian, advisor
dc.contributor.authorKennan, Alan, committee member
dc.contributor.authorCrans, Debbie, committee member
dc.contributor.authorReynolds, Melissa, committee member
dc.contributor.authorDi Pietro, Santiago, committee member
dc.date.accessioned2007-01-03T05:57:16Z
dc.date.available2017-01-31T06:30:24Z
dc.date.issued2014
dc.description.abstractProteinaceous reagents, including antibodies and synthetic proteins, have become some of the most effective reagents for targeted treatment and diagnosis of disease. The unique catalytic activity of some proteins and ability to bind disease-relevant receptors that can evade small molecule discovery, make these reagents well suited for use as therapeutic and bioimaging reagents. However, the large size and charge distribution of most proteins greatly inhibits their intracellular delivery to diseased cells, limiting targets to those displayed on the cell surface. In response to this challenge, we have developed a bacteriophage nanocarrier to deliver large payloads of proteinaceous cargo to the interior of prostate cancer cells. This reagent employs two distinct components: a genetically defined prostate cancer cell-selective protein transduction domain, and a biotinylation site on an orthogonal coat protein, which allows for complexation with streptavidin fusion proteins. Collectively, this approach permits targeted intracellular delivery of ~20 exogenous proteins per phage to human prostate cancer cells. This multifunctional technology offers a cell-selective solution to the challenges associated with delivering protein cargo to the interior of diseased cells and may lead to an expansion in the use of protein reagents.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierDePorter_colostate_0053A_12661.pdf
dc.identifier.urihttp://hdl.handle.net/10217/88422
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleEngineering bacteriophage nanocarriers for targeted delivery of protein reagents to prostate cancer cells
dc.typeText
dcterms.embargo.expires2017-01-31
dcterms.embargo.terms2017-01-31
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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