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Hemocompatibility of polymeric materials for blood-contacting applications

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dc.contributor.authorWoodbury, Jodi Marie, author
dc.contributor.authorPopat, Ketul, advisor
dc.contributor.authorDasi, Prasad, committee member
dc.contributor.authorReynolds, Melissa, committee member
dc.date.accessioned2015-08-27T03:57:24Z
dc.date.available2015-08-27T03:57:24Z
dc.date.issued2015
dc.description.abstractHemocompatibility of a biomaterial plays a vital role in the overall success of the biomaterial in the body. Every implanted biomaterial tends to cause an immune response by the host tissue. The intensity of said response depends on many factors, including the properties of the material itself. In this study, we have assessed the hemocompatibility of expanded polytetrafluoroethylene (ePTFE), linear low-density polyethylene (LLDPE) and polyethylene terephthalate (PET); 3 potential materials for blood-contacting applications. The surface morphology was characterized using scanning electron microscopy (SEM), and surface wettability was characterized using contact angle goniometry. The cytotoxicity was investigated using lactate dehydrogenase (LDH) assay. The adsorption of key blood serum proteins was evaluated using micro-bicinchoninic acid (micro-BCA) assay. The results were visualized using SEM. Platelet adhesion and activation was investigated using live cell staining and SEM. Whole blood clotting kinetics were evaluated using a hemolysis assay and the results visualized using SEM. The results indicate that none of these materials are cytotoxic. Protein adsorption was highest on PET, and platelet adhesion was significantly higher on PET. However, the percentage of activated platelets and whole blood clotting kinetics was comparable on all materials. This work successfully creates a baseline against which the hemocompatibility of modified ePTFE, LLDPE and PET can be measured.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierWoodbury_colostate_0053N_12983.pdf
dc.identifier.urihttp://hdl.handle.net/10217/167017
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectheart valve
dc.subjectimplant
dc.subjectbiomaterial
dc.subjectplatelets
dc.subjecthemocompatibility
dc.titleHemocompatibility of polymeric materials for blood-contacting applications
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBioengineering
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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