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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples

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dc.contributor.authorMcPeters, Ryley Adam, author
dc.contributor.authorDooley, Gregory, advisor
dc.contributor.authorHanneman, William, advisor
dc.contributor.authorBorch, Thomas, committee member
dc.date.accessioned2007-01-03T08:34:16Z
dc.date.available2007-01-03T08:34:16Z
dc.date.issued2012
dc.description.abstractA method was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification and confirmation of 8 drugs of abuse (cocaine, codeine, MDMA, methadone, methamphetamine, morphine, nicotine, and oxycodone) and their various metabolites (acetylmorphine, cotinine, EDDP, amphetamine and benzoylecgonine) in municipal wastewater effluent samples. Samples were collected once daily and drugs were extracted from the wastewater with solid phase extraction using Waters Oasis MCX cartridges. Ultra-high-pressure liquid chromatography (UPLC) and positive electrospray ionization was used along with dynamic multiple reaction monitoring (MRM) tandem mass spectrometry for identification and quantitation. The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with recoveries ranging from 70%-140%. Deuterated internal standards for each analyte were used to correct for matrix effects, ion suppression, and sample preparation errors. The validated method was applied to municipal wastewater samples collected from a point source effluent into Fossil Creek, Fort Collins, CO. Eight of thirteen drugs being measured were found on a daily basis with the maximum being 307.72 mg/min of benzoylecgonine. Samples showed various spikes in drug concentration at 7 day intervals that corresponded with weekends.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierMcPeters_colostate_0053N_11453.pdf
dc.identifierETDF2012400435ERHS
dc.identifier.urihttp://hdl.handle.net/10217/74426
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectdrugs of abuse
dc.subjectwastewater
dc.subjectmetabolites
dc.subjectLC-MS/MS
dc.titleLC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineEnvironmental and Radiological Health Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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