Show simple item record

dc.contributor.advisorMcNaughton, Brian
dc.contributor.authorBlakeley, Brett D.
dc.contributor.committeememberKennan, Alan
dc.contributor.committeememberFisk, Nick
dc.contributor.committeememberReynolds, Melissa
dc.contributor.committeememberPeersen, Olve
dc.date.accessioned2007-01-03T05:57:12Z
dc.date.available2007-01-03T05:57:12Z
dc.date.issued2014
dc.description2014 Fall.
dc.descriptionIncludes bibliographical references.
dc.description.abstractPotent and selective recognition of disease-relevant macromolecules - such as proteins and RNA - is the molecular basis of most pharmaceuticals . Historically, small (< 500 Da) molecules have filled this role. However, the overwhelming majority (~85%) of the proteome - and emerging therapeutic targets such as RNA - present a serious challenge to small molecule-dependent recognition. An alternative approach to potent and selective recognition and regulation of disease-relevant proteins and RNA is to use synthetic proteins. In contrast to small molecules, the size, relatively high folding energies (>10 kcal/mol) and functional group diversity (by virtue of proteinaceous amino acids) allow proteins to recognize - and potentially control - macromolecular receptors that evade small molecules. Presented here are two approaches to advancing the discovery of new proteins that recognize either disease-relevant protein or RNA targets. The first part of this thesis describes split superpositive GFP reassembly as a method to identify novel protein-protein interacting pairs in living cells (E. coli). The second part of this thesis describes basic studies to evaluate the suitability of a naturally occurring RNA Recognition Motif (RRM) as a scaffold for targeting disease-relevant RNA hairpins, and the development of new RRMs that target TAR RNA, a hairpin critical to HIV proliferation.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierBlakeley_colostate_0053A_12669.pdf
dc.identifier.urihttp://hdl.handle.net/10217/88415
dc.languageEnglish
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019 - CSU Theses and Dissertations
dc.rightsCopyright of the original work is retained by the author.
dc.titleMethods for detecting and developing protein-protein or protein-RNA interactions
dc.typeText
dcterms.rights.dplaThe copyright and related rights status of this item has not been evaluated (https://rightsstatements.org/vocab/CNE/1.0/). Please refer to the organization that has made the Item available for more information.
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record