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Methods for detecting and developing protein-protein or protein-RNA interactions

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dc.contributor.authorBlakeley, Brett D., author
dc.contributor.authorMcNaughton, Brian, advisor
dc.contributor.authorKennan, Alan, committee member
dc.contributor.authorFisk, Nick, committee member
dc.contributor.authorReynolds, Melissa, committee member
dc.contributor.authorPeersen, Olve, committee member
dc.date.accessioned2007-01-03T05:57:12Z
dc.date.available2007-01-03T05:57:12Z
dc.date.issued2014
dc.description.abstractPotent and selective recognition of disease-relevant macromolecules - such as proteins and RNA - is the molecular basis of most pharmaceuticals . Historically, small (< 500 Da) molecules have filled this role. However, the overwhelming majority (~85%) of the proteome - and emerging therapeutic targets such as RNA - present a serious challenge to small molecule-dependent recognition. An alternative approach to potent and selective recognition and regulation of disease-relevant proteins and RNA is to use synthetic proteins. In contrast to small molecules, the size, relatively high folding energies (>10 kcal/mol) and functional group diversity (by virtue of proteinaceous amino acids) allow proteins to recognize - and potentially control - macromolecular receptors that evade small molecules. Presented here are two approaches to advancing the discovery of new proteins that recognize either disease-relevant protein or RNA targets. The first part of this thesis describes split superpositive GFP reassembly as a method to identify novel protein-protein interacting pairs in living cells (E. coli). The second part of this thesis describes basic studies to evaluate the suitability of a naturally occurring RNA Recognition Motif (RRM) as a scaffold for targeting disease-relevant RNA hairpins, and the development of new RRMs that target TAR RNA, a hairpin critical to HIV proliferation.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierBlakeley_colostate_0053A_12669.pdf
dc.identifier.urihttp://hdl.handle.net/10217/88415
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleMethods for detecting and developing protein-protein or protein-RNA interactions
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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Methods for detecting and developing protein-protein or protein-RNA interactions
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