From RNA-Seq to gene annotation using the splicegrapher method
Messenger RNA (mRNA) plays a central role in carrying out the instructions encoded in a gene. A gene's components may be combined in various ways to generate a diverse range of mRNA molecules, or transcripts, through a process called alternative splicing (AS). This allows each gene to produce different products under different conditions, such as different stages of development or in different tissues. Researchers can study the diverse set of transcripts a gene produces by sequencing its mRNA. The latest sequencing technology produces millions of short sequence reads (RNA-Seq) from mRNA transcripts, ...
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