Evaluation of a bioassay method for thyroid stimulating hormone and its application to Citellus richardsoni, An
An in vitro bioassay for thyroid stimulating hormone (TSH) based upon the TSH-stimulated release of I131 was evaluated. Significant error was not introduced by the method of pipetting aliquots of incubation medium in order to estimate I131 activity. Incubation times for uptake and release agree with those suggested by Bottari's group. While per cent uptake and absolute release of I131 were significantly related to the weight of tissue incubated, per cent release was not, and weighing of tissue before incubation was not necessary. It was found that storage of TSH in plasma at -10° C did not ...
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