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An evaluation of a bioassay method for thyroid stimulating hormone and its application to Citellus richardsoni

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dc.contributor.authordeGraw, William A., author
dc.date.accessioned2007-01-03T08:10:11Z
dc.date.available2007-01-03T08:10:11Z
dc.date.issued1965
dc.description.abstractAn in vitro bioassay for thyroid stimulating hormone (TSH) based upon the TSH-stimulated release of I131 was evaluated. Significant error was not introduced by the method of pipetting aliquots of incubation medium in order to estimate I131 activity. Incubation times for uptake and release agree with those suggested by Bottari's group. While per cent uptake and absolute release of I131 were significantly related to the weight of tissue incubated, per cent release was not, and weighing of tissue before incubation was not necessary. It was found that storage of TSH in plasma at -10° C did not preserve TSH activity, thus fresh samples were used. U.S.P. reference standard TSH was quantitatively recovered from horse serum, rat plasma, and Richardson ground squirrel plasma, with no apparent inhibition and with the linear range of the log-dose response lying between 5--200 ImU/100 mI. Assay results are reported for male Holtzman strain rats. C. richardsoni plasma from summer and winter squirrels and one spring squirrel was assayed with the following results which support indirect findings of other investigators: summer, 34 ImU/100 ml; winter, 4 ImU/100 ml; spring, 116 ImU/100 mI.
dc.format.mediummasters theses
dc.identifier1965_Spring_deGraw_William.pdf
dc.identifierETDF1965100003
dc.identifier.urihttp://hdl.handle.net/10217/67616
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relationCatalog record number (MMS ID): 991003739559703361
dc.relationRS190.H6.D4
dc.relation.ispartof1950-1979
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectBiological assay
dc.subjectRichardson's ground squirrel
dc.subjectHormones -- Research
dc.titleAn evaluation of a bioassay method for thyroid stimulating hormone and its application to Citellus richardsoni
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplinePhysiology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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