Effect of histone H3 E73D mutation on in vitro chromatin silencing
Organization of DNA into chromatin requires the presence of small basic proteins called histones. Core histones are highly conserved across various species. In addition to their function as DNA packing material, histones play an important role in the regulation of transcription, replication, and gene silencing. In particular, histone-mediated silencing is achieved via histone modifications and through interactions with suppressor proteins. In the yeast Saccharomyces cerevisiae, a single amino acid substitution (E73D) found within the conserved region of histone H3, has been shown to de-repress ...
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