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Effect of histone H3 E73D mutation on in vitro chromatin silencing

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dc.contributor.authorColbert, Karen, author
dc.contributor.authorSubramanian, Vidya, author
dc.contributor.authorThompson, Jeffrey, author
dc.contributor.authorLuger, Karolin, author
dc.date.accessioned2007-01-03T05:52:36Z
dc.date.available2007-01-03T05:52:36Z
dc.date.issued2007
dc.description.abstractOrganization of DNA into chromatin requires the presence of small basic proteins called histones. Core histones are highly conserved across various species. In addition to their function as DNA packing material, histones play an important role in the regulation of transcription, replication, and gene silencing. In particular, histone-mediated silencing is achieved via histone modifications and through interactions with suppressor proteins. In the yeast Saccharomyces cerevisiae, a single amino acid substitution (E73D) found within the conserved region of histone H3, has been shown to de-repress silencing at the telomeres and mating type loci in yeast (Thompson et al., 2003). It has been proposed that the substitution may shorten the amino acid side chain length enough to disrupt a necessary interaction at the H3-H4 interface within the nucleosome. Alternatively, the E73D mutation may affect the binding affinity of Sir3 for histone H3. Sir3 and Sir4, chromatin-associated repressor proteins, are known to mediate telomeric and mating loci silencing in S. cerevisiae (Grunstein et al., 1997). In order to test the effect of the E73D mutation on nucleosome stability in vitro, the crystal structure of nucleosomes containing the H3 mutant will be determined to ascertain the charge interactions between the amino acid residues within the vicinity of the substituted residue. In addition, binding studies will be carried out to investigate Sir protein binding to nucleosomes containing H3 E73D histones. Finally, analytical ultracentrifugation experiments will provide quantitative data on the in vitro dynamics of nucleosomal arrays comprised of nucleosomes containing H3 E73D.
dc.description.awardHighest Honors.
dc.format.mediumStudent works
dc.format.mediumposters
dc.identifier.urihttp://hdl.handle.net/10217/549
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2007 Projects
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjecthistones
dc.subjectDNA
dc.titleEffect of histone H3 E73D mutation on in vitro chromatin silencing
dc.typeStillImage
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineNatural Sciences
thesis.degree.disciplineBiochemistry and Molecular Biology

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Effect of Histone H3 E73D Mutation on in vitro Chromatin Silencing
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