M-CSF and GM-CSF influence naïve murine alveolar macrophage differentiation and function in vitro
Date
2008
Authors
Higgins, Jonathan R., author
Higgins, David M., author
Lee, Eric J., author
Orme, Ian M., author
Gonzalez-Juarrero, Mercedes, author
Journal Title
Journal ISSN
Volume Title
Abstract
The objective of this study was to compare the effect of M-CSF and GM-CSF in their abilities to affect the functional and phenotypical characteristics of AMs. In this study, we harvested naïve uninfected murine alveolar macrophages by bronchoalveolar lavage (BAL) and cultured in the presence of M-CSF, GM-CSF or both. GM-CSF stimulated cell proliferation at a higher rate when measured by relative loss of CFDA-SE dye and had higher phagocytic capacity than M-CSF cultured cells. In contrast, alveolar macrophages cultured in the presence of M-CSF exhibited a dendritic-cell-like morphology and upregulated expression of dendriticcell- associated markers like CCR7, MHC II and DEC205. The dendritic cell character of M-CSF-treated AMs was also manifested in their higher capacity to stimulate CD4+ T cell proliferation in a mixed lymphocyte reaction (MLR). This study demonstrates that M-CSF has a strong ability to differentiate AMs into DC-like cells.
Description
This research has been published in Relative Levels of M-CSF and GM-CSF Influence the Specific Generation of Macrophage Populations during Infection with Mycobacterium tuberculosis by Higgins, D et al. in Journal of Immunology, 2008, 180: 4892-4900.
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Subject
M-CSF
GM-CSF
alveolar macrophages
AM
dendritic cells
DC