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Protective effects of sulforaphane on nitric oxide induced mitochondrial dysfunction

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dc.contributor.authorAcerbo, Evan R., author
dc.contributor.authorHanneman, William, advisor
dc.contributor.authorLegare, Marie, committee member
dc.contributor.authorChicco, Adam, committee member
dc.date.accessioned2019-06-14T17:06:41Z
dc.date.available2020-06-10T17:05:25Z
dc.date.issued2019
dc.description.abstractSulforaphane (SFN), an isothiocyanate compound that is formed in the breakdown process of cruciferous vegetables, has demonstrated the ability to interfere with dynamin-related protein 1 (Drp1)-mediated mitochondrial fission. The present study investigated whether SFN can protect cells exhibiting persistent mitochondrial fission induced by nitrosative stress (S-nitrosoglutathione; GSNO), and shed light on the mechanism by which this occurs. Results show that SFN (5 μM) prevents decreases in the rate of mitochondrial oxidative phosphorylation (ATP production) in SH-SY5Y neuroblastoma cells treated with 200-600 μM GSNO, which was associated with significant improvements in cell viability at all doses. Based upon the understood activation mechanism of Drp1, we further explored the possibility that SFN interferes with phosphorylation of Drp1 at serine residue 616 (pDrp1-Ser616). Indeed, SFN significantly reduced GSNO-mediated increases in pDrp1-Ser616, suggesting a possible mechanism of cytoprotection. However, due to the various reported targets of SFN, it remains unclear if SFN interferes directly with Drp1 phosphorylation or with other targets upstream of this event.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierAcerbo_colostate_0053N_15426.pdf
dc.identifier.urihttps://hdl.handle.net/10217/195379
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleProtective effects of sulforaphane on nitric oxide induced mitochondrial dysfunction
dc.typeText
dcterms.embargo.expires2020-06-10
dcterms.embargo.terms2020-06-10
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineEnvironmental and Radiological Health Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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