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The effect of lipid and oxygen concentration on myosin heavy chain isoform expression in c2c12 and cultured Weddell seal (Leptonychotes weddellii) skeletal muscle cells

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dc.contributor.authorGarcia, Teresa Maria, author
dc.contributor.authorKanatous, Shane, advisor
dc.contributor.authorHamilton, Karyn, committee member
dc.contributor.authorMykles, Don, committee member
dc.date.accessioned2007-01-03T06:39:24Z
dc.date.available2007-01-03T06:39:24Z
dc.date.issued2014
dc.description.abstractThe Weddell seal (Leptonychotes weddellii), a diving mammal, has large stores of myoglobin as compared to terrestrial mammals. Understanding the regulation of myoglobin in this unique species has therapeutic potential in people at risk of muscle infarction; to pursue this end, we established a successful protocol for the isolation and culture of primary Weddell seal skeletal muscle cells utilizing lipid-supplemented culture media. Using these cells, a study was designed to determine if increasing lipid in the media in either normoxic or hypoxic conditions would affect the expression of myosin heavy chain (MHC), which might explain a previously observed increase in myoglobin expression with increased lipid. Gel electrophoresis consistently resolved two putative MHC isoforms from seal and c2c12 cells. LC-MS/MS determined that the resolved bands were heterogeneous, and that all bands contained minimal adult MHC (10% or less, as measured by NSAF). Despite the minimal expression of adult MHC isoforms, myoglobin was detected in each treatment group. This study suggests that electrophoresis methods alone may not be sufficient to detect changes in MHC isoform abundance in cell culture, and that, in cell culture, regulatory pathways may exist for myoglobin that are independent of adult fiber type.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierGarcia_colostate_0053N_12519.pdf
dc.identifier.urihttp://hdl.handle.net/10217/83965
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectfiber type
dc.subjectlipid
dc.subjectmyoblast
dc.subjectmyoglobin
dc.subjectprimary cell culture
dc.subjectWeddell seal
dc.titleThe effect of lipid and oxygen concentration on myosin heavy chain isoform expression in c2c12 and cultured Weddell seal (Leptonychotes weddellii) skeletal muscle cells
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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