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Dataset associated with "mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage"

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dc.contributor.authorParker, Dylan, M.
dc.contributor.authorWinkenbach, Lindsay, P.
dc.contributor.authorBoyson, Samuel, P.
dc.contributor.authorSaxton, Matthew, N.
dc.contributor.authorDaidone, Camryn
dc.contributor.authorAl-Mazaydeh, Zainab, A.
dc.contributor.authorNishimura, Marc, T.
dc.contributor.authorMueller, Florian
dc.contributor.authorOsborne Nishimura, Erin
dc.coverage.spatialColorado State University, Fort Collins (Colo.)en_US
dc.coverage.temporal2016-2020en_US
dc.date.accessioned2020-03-27T20:27:28Z
dc.date.available2020-03-27T20:27:28Z
dc.date.issued2020
dc.descriptionThis dataset contains all primary microscopy data presented or analyzed in the publication "mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage." Source data and code used for creation of plots can be found at https://github.com/muellerflorian/parker-rna-loc-elegans.en_US
dc.descriptionDepartment of Biology
dc.descriptionDepartment of Biochemistry & Molecular Biology
dc.description.abstractCaenorhabditis elegans early embryos generate cell-specific transcriptomes despite lacking active transcription. This presents an opportunity to study mechanisms of post-transcriptional regulatory control. In seeking the mechanisms behind this patterning, we discovered that some cell-specific mRNAs accumulate non-homogenously within cells, localizing to membranes, P granules (associated with progenitor germ cells in the P lineage), and P-bodies (associated with RNA processing). Transcripts differed in their dependence on 3'UTRs and RNA Binding Proteins, suggesting diverse regulatory mechanisms. Notably, we found strong but imperfect correlations between low translational status and P granule localization within the progenitor germ lineage. By uncoupling these, we untangled a long-standing question: Are mRNAs directed to P granules for translational repression or do they accumulate there as a downstream step? We found translational repression preceded P granule localization and could occur independent of it. Further, disruption of translation was sufficient to send homogenously distributed mRNAs to P granules. Overall, we show transcripts important for germline development are directed to P granules by translational repression, and this, in turn, directs their accumulation in the progenitor germ lineage where their repression can ultimately be relieved.en_US
dc.description.sponsorshipNational Institutes of Health R35GM124877.en_US
dc.description.sponsorshipBoettcher Webb-Waring.en_US
dc.description.sponsorshipNSF GAUSSI training grant (DGE-1450032).en_US
dc.description.sponsorshipSome funding through Institut Pasteur.en_US
dc.format.mediumBZIP2
dc.format.mediumTXT
dc.format.mediumTIFF
dc.identifier.urihttps://hdl.handle.net/10217/201623
dc.identifier.urihttp://dx.doi.org/10.25675/10217/201623
dc.languageEnglishen_US
dc.language.isoengen_US
dc.publisherColorado State University. Librariesen_US
dc.relation.ispartofResearch Data
dc.relation.isreferencedbyParker, D. M., Winkenbach, L. P., Boyson, S., Saxton, M. N., Daidone, C., Al-Mazaydeh1, Z. A., Nishimura, M. T., Mueller, F., & Nishimura, E. O. (2020). mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage. Development. http://dx.doi.org/10.1242/dev.186817en_US
dc.rights.licenseThis data is open access and distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectmRNA localization
dc.subjectC. elegans
dc.subjectP granule
dc.titleDataset associated with "mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage"en_US
dc.typeDataseten_US

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