Influence of management practices on virulence factors, antimicrobial resistance genes and heavy metal resistance genes in broiler chicken production
| dc.contributor.author | Woyda, Reed Richard, author | |
| dc.contributor.author | Abdo, Zaid, advisor | |
| dc.contributor.author | Oladeinde, Adelumola, committee member | |
| dc.contributor.author | Daniels, Josh, committee member | |
| dc.contributor.author | Sloan, Dan, committee member | |
| dc.contributor.author | Stenglein, Mark, committee member | |
| dc.date.accessioned | 2023-06-01T23:55:48Z | |
| dc.date.available | 2023-06-01T23:55:48Z | |
| dc.date.issued | 2023 | |
| dc.description | Zip file contains supplementary tables and figures. | |
| dc.description.abstract | The main bacterial species associated with food-borne illness in humans are Escherichia coli, Salmonella species and Campylobacter species. The ability of a bacterial strain to survive the food-production pipeline and to mount an infection and cause disease in humans is dependent on an array of genetic factors. The presence of specific virulence factors will influence the severity of disease while antimicrobial resistance genes affect the choice and efficacy of treatment. Management practices in poultry production aim at reducing the incidence of poultry and human bacterial pathogens and, in general, at maintaining a healthy flock and a healthy global population. However, the influence of management practices, in a post-antibiotic era, on pathogenic bacterial species, and in particular the selective pressures imposed on genetic factors such as antimicrobial and metal resistance and virulence factors, are understudied. In Chapter 2, we provide a robust bacterial genomic analysis pipeline which is used for subsequent analysis in the following chapters. Chapter 3 provides an understanding of the current antimicrobial resistance and virulence factors present in chicken production and human clinical settings. This work found these host sources harbored different antimicrobial resistance genes and virulence factors that can classify them into phylogroups and host origin. In Chapter 4, through characterization of Campylobacter species isolated from broiler litter, we determined the reused litter environment selected for Campylobacter species lacking virulence factors aiding in colonization of chicken and human hosts. In Chapter 5, we determined the practice of adding copper sulfate to drinking water, commonly used for growth promotion or sanitization, may have selected for, and provided a reservoir for, Salmonella strains harboring plasmid-borne copper resistance genes. Overall, this work provides a computational pipeline for the high-throughput analysis of bacterial genomes and provides insights into selective pressures imposed on pathogenic bacterial species by modern-day management practices. | |
| dc.format.medium | born digital | |
| dc.format.medium | doctoral dissertations | |
| dc.format.medium | ZIP | |
| dc.format.medium | XLSX | |
| dc.format.medium | PNG | |
| dc.identifier | woyda_colostate_0053A_17612.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/236648 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.title | Influence of management practices on virulence factors, antimicrobial resistance genes and heavy metal resistance genes in broiler chicken production | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Cell and Molecular Biology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy (Ph.D.) |