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Detection of disease biomarkers using a novel multi-analyte immunoassay

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dc.contributor.authorCaulum, Meghan M., author
dc.contributor.authorHenry, Charles S., advisor
dc.date.accessioned2024-03-13T18:50:56Z
dc.date.available2024-03-13T18:50:56Z
dc.date.issued2007
dc.description.abstractBiomarkers provide clinicians with an important tool for disease assessment. Many different biomarkers have been discovered making it readily apparent that no single biomarker can be relied upon for accurate disease detection which has led towards the push for new multi-analyte screening methods. There are, however, currently few inexpensive multi-analyte methods that can use these biomarkers for disease detection in a point-of-care setting. This dissertation details the development of a novel immunoassay which bridges the gap between traditional immunoassays and high density microarrays by utilizing microfluidics, immunoassays and micellar electrokinetic chromatography (MEKC). This chemistry, the Cleavable Tag Immunoassay (CTI), is a low- to medium-density heterogeneous immunoassay designed to detect up to 20 analytes simultaneously. The multi-analyte CTI is shown to be a useful tool for the detection and quantification of cardiac biomarkers in serum samples. Limit of detection and linear range are reported for all of the biomarkers with LODs on the order of low ng/mL to low pg/mL. The linear range for each of the biomarkers spans the boundary between normal and elevated levels. In addition, the use of a combination of two surfactants in the run buffer which form mixed micelles is shown to improve resolution of CTI tags. Preliminary studies of cleavage kinetics using single particles for an integrated CTI analyzer are also presented including demonstration of the ability to trap and release magnetic particles in a microchip device. Two methods for improving separation efficiency of poly(dimethylsiloxane) including the use of polyelectrolyte multilayers and a simple and effective way to generate a stable hydrophilic glass-like surface for use with microchip CE-EC and CE-fluorescence are also presented. The outcome of this dissertation demonstrates the marriage of immunoassays and microchip MEKC in the CTI shows promise as a new medium-density chemistry for rapid biomarker detection. This work is the first step towards the development of an integrated assay for rapid point-of-care analysis of AMI.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierETDF_Caulum_2007_3266402.pdf
dc.identifier.urihttps://hdl.handle.net/10217/237630
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.rights.licensePer the terms of a contractual agreement, all use of this item is limited to the non-commercial use of Colorado State University and its authorized users.
dc.subjectbiomarkers
dc.subjectcleavable tags
dc.subjectimmunoassays
dc.subjectanalytical chemistry
dc.titleDetection of disease biomarkers using a novel multi-analyte immunoassay
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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