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The molecular ecology and evolution of puma letivirus in bobcats and mountain lions in North America

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dc.contributor.authorLee, Justin S., author
dc.contributor.authorVandeWoude, Sue, advisor
dc.contributor.authorCrooks, Kevin R., advisor
dc.contributor.authorFunk, W. Chris, committee member
dc.contributor.authorTroyer, Jennifer L., committee member
dc.date.accessioned2007-01-03T05:53:48Z
dc.date.available2007-01-03T05:53:48Z
dc.date.issued2013
dc.description.abstractHost-pathogen dynamics are influenced by ecological and evolutionary processes at all levels of biological organization. Within individuals, viruses that cause chronic infection must either avoid or escape the pressures of the host immune system. Furthermore, viruses adapted to one host environment may have low fitness when transmitted to different individuals, populations, and species. At the landscape level, the movement and distribution of directly transmitted obligate pathogens are inextricably associated with their hosts. We used molecular analyses to investigate the ecology and evolution of feline immunodeficiency virus (FIV) in bobcats and mountain lions within individuals, among populations, and between species of hosts. In Chapter One we investigated the effects of urban development on the movement of bobcats and feline immunodeficiency virus (FIV) among a fragmented landscape in southern California. Our results demonstrate that bobcat movement and gene flow are restricted across a major freeway that bisects the study area, resulting in two genetically and physically distinct subpopulations connected by a low level of migration. However, the FIV population is not similarly structured, suggesting that movements and contacts sufficient for disease transmission continue despite the low level of host migration observed. Chapter Two investigates the causes and effects of FIV evolution among bobcats and mountain lions across North America. Our results illustrate a dynamic host-pathogen relationship characterized by host-immune pressures and a rapidly evolving virus with a highly plastic genome. Finally, in Chapter Three we describe a pilot project aimed at improving the efficiency with which pathogen genetic data can be collected by combining the use of two modern technologies - targeted genome capture and next-generation sequencing. The results suggest this is a promising approach to detecting and sequencing multiple pathogens from biological samples. Collectively, the work described in this dissertation combines new and existing methodologies to generate, analyze, and interpret molecular data to answer complex questions about the ecological and evolutionary determinants of host-pathogen dynamics.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierLee_colostate_0053A_11783.pdf
dc.identifier.urihttp://hdl.handle.net/10217/80159
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectdisease ecology
dc.subjectfeline immunodeficiency virus
dc.subjectpuma concolor
dc.subjectpuma lentivirus
dc.subjectviral evolution
dc.subjectLynx rufus
dc.titleThe molecular ecology and evolution of puma letivirus in bobcats and mountain lions in North America
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMicrobiology, Immunology, and Pathology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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