Dataset associated with "The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release"
Date
2020
Authors
Shields, Mallory
Bowers, Matthew
Kramer, Hannah
Fulcer, McKenzie
Perinet, Lara
Metz, Marissa
Reist, Noreen
Journal Title
Journal ISSN
Volume Title
Abstract
Following nerve stimulation, there are two distinct phases of Ca2+-dependent neurotransmitter release: a fast, synchronous release phase, and a prolonged, asynchronous release phase. Each of these phases is tightly regulated and mediated by distinct mechanisms. Synaptotagmin 1 is the major Ca2+ sensor that triggers fast, synchronous neurotransmitter release upon Ca2+ binding by its C2A and C2B domains. It has also been implicated in the inhibition of asynchronous neurotransmitter release, as blocking Ca2+ binding by the C2A domain of synaptotagmin 1 results in increased asynchronous release. However, the mutation used to block Ca2+ binding in the previous experiments (aspartate to asparagine mutations, sytD-N) had the unintended side effect of mimicking Ca2+ binding, raising the possibility that the increase in asynchronous release was directly caused by ostensibly constitutive Ca2+ binding. Thus, rather than modulating an asynchronous sensor, sytD-N may be mimicking one. To directly test this C2A inhibition hypothesis, we utilized an alternate C2A mutation that we designed to block Ca2+ binding without mimicking it (an aspartate to glutamate mutation, sytD-E). Analysis of both the original sytD-N mutation and our alternate sytD-E mutation at the Drosophila neuromuscular junction showed differential effects on asynchronous release, as well as on synchronous release and the frequency of spontaneous release. Importantly, we found that asynchronous release is not increased in the sytD-E mutant. Thus, our work provides new mechanistic insight into synaptotagmin 1 function during Ca2+-evoked synaptic transmission and demonstrates that Ca2+ binding by the C2A domain of synaptotagmin 1 does not inhibit asynchronous neurotransmitter release in vivo.
Description
This dataset includes all raw data for experiments included in the manuscript. It also includes raw, unedited microscopy and blot images.
College of Veterinary Medicine and Biomedical Sciences
Department of Biomedical Sciences
College of Veterinary Medicine and Biomedical Sciences
Department of Biomedical Sciences
Rights Access
Subject
synapse
neuroscience
vesicle fusion
neurotransmitter release
synaptotagmin
asynchronous release
Citation
Associated Publications
Shields MC, Bowers MR, Kramer HL, Fulcer MM, Perinet LC, Metz MJ, et al. (2020) The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release. PLoS ONE 15(5): e0232991. https://doi.org/10.1371/journal.pone.0232991