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Are you what you eat? Assessing intracellular effects of supplemental lipid within mouse (C2C12) skeletal muscle cells

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dc.contributor.authorMontano, Dominique, author
dc.contributor.authorKanatous, Shane, advisor
dc.contributor.authorBell, Christopher, advisor
dc.contributor.authorEngle, Terry, committee member
dc.contributor.authorFlorant, Gregory, committee member
dc.date.accessioned2021-09-06T10:25:17Z
dc.date.available2021-09-06T10:25:17Z
dc.date.issued2021
dc.description.abstractThe expression of myoglobin, an oxygen-binding protein, facilitates aerobically-powered dives by effectively distributing a finite oxygen supply during breath-hold dives in marine mammals (Costa 2004, Kanatous et al. 1999, Wright 2006). Multiple factors have been experimentally shown to contribute to increasing myoglobin expression, thus influencing aerobic capacity. Among the known expression pathways, the most recent and least studied involves a link between lipid supplementation and increased myoglobin expression. We are unaware of the specific conditions that drive this expression, and whether or not fatty acids play an intracellular role in upregulating myoglobin. This work elucidates the effects of lipid supplementation on the expression of myoglobin in terrestrial mammals, as well as enhances our understanding of cues that initiate the expression of factors contributing to the positive development of aerobically-based exercise in the whole animal. In order to understand these lipid influences on an aerobically-adapted animal such as a marine mammal, we focused on developing experimental designs and applications geared towards a model mammalian system of C2C12 mouse cells. Lipids possess the capacity to influence aerobic ability in mammalian models, whether through marine mammals' reliance on lipid metabolism to power aerobically-based exercise, regulating mitochondrial respiration, fatty acid metabolism that generates increased ATP from oxidative phosphorylation relative to metabolic pathways like glycolysis, lipids acting as ligands to affect genes that contribute to aerobic capacity, or lipid supplementation that increases myoglobin expression. Multiple studies from our lab have shown that lipid present in the media upregulates myoglobin in C2C12 cells, driving expression of the protein in conjunction with other elements. Although we have determined a link between lipid supplementation and myoglobin expression, the particular intracellular effects ranging from uptake, transporter protein regulation, and either storage, metabolism, or specific effects directly affecting myoglobin expression still remain to be uncovered. The factors and conditions that regulate myoglobin expression are extensive, and lipids as an avenue to myoglobin expression is a relatively new exploration that will have applications for numerous areas of physiological research. This work has applications in multiple mammalian models, including humans, and will serve to enhance our understanding of the factors that drive aerobically-based endurance exercise, as well as understanding the cues and stimuli required to increase expression of key factors that regulate aerobic capacity in mammalian systems.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierMontano_colostate_0053N_16771.pdf
dc.identifier.urihttps://hdl.handle.net/10217/233759
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectcell culture
dc.subjectmouse
dc.subjectseals
dc.subjectmarine mammals
dc.subjectc2c12
dc.subjectmyoglobin
dc.titleAre you what you eat? Assessing intracellular effects of supplemental lipid within mouse (C2C12) skeletal muscle cells
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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