Gene expression analysis before and after the pelvic flexure in the equine hindgut
| dc.contributor.author | Moss, Cameron D., author | |
| dc.contributor.author | Coleman, Stephen J., advisor | |
| dc.contributor.author | Engle, Terry, committee member | |
| dc.contributor.author | Metcalf, Jessica, committee member | |
| dc.contributor.author | Landolt, Gabriele, committee member | |
| dc.date.accessioned | 2024-05-27T10:31:43Z | |
| dc.date.available | 2024-05-27T10:31:43Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | The equine hindgut is the primary site of the horse's nutrient breakdown, absorption, and energy production. More than 60% of the horse's energy comes from hindgut fermentation. In this process, commensal microbes in the hindgut aid in the digestion of plant materials to create volatile fatty acids that can be used by host cells to make energy. Many severe health issues- such as colic, laminitis, or colonic impactions- often occur in the equine hindgut, making it an important site to study to provide better management, treatment, and prevention options for horses suffering from gastrointestinal disease. Although much research exists focusing on the microbiome and overall physiology of the equine hindgut, relatively little addresses the role of gene expression in maintaining a complex yet essential homeostatic balance within the gastrointestinal tract. Previous from our lab found major differences in the microbial content of gastrointestinal compartments of the equine hindgut, separated by the pelvic flexure. The pelvic flexure is a short, narrow, horseshoe-shaped loop in the equine large colon. It defines the ventral and dorsal segments of the colon and is a common site of colonic impaction in horses. Although the pelvic flexure cannot and should not act as a "barrier," something "barrier-like" may be occurring around this region as it pertains to the hindgut microbiome. The mechanism for this action is not defined. As a result, this thesis aims to investigate gene expression in the intestinal epithelial cells of the ventral colon, pelvic flexure, and dorsal colon regions of a healthy hindgut to determine what differences exist. The insight gained from this analysis will provide a baseline for comparison to understand how gene expression patterns in these tissues adapt to changes in the microbiome and external factors like diet. The results of this thesis are the first steps towards a better understanding of homeostasis in the equine hindgut. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier | Moss_colostate_0053N_18175.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/238354 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | equine hindgut | |
| dc.subject | microbiome | |
| dc.subject | physiology | |
| dc.subject | genetics | |
| dc.subject | bioinformatics | |
| dc.subject | pelvic flexure | |
| dc.title | Gene expression analysis before and after the pelvic flexure in the equine hindgut | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Animal Sciences | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
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