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The curious case of chemotaxis in soft rot Pectobacteriaceae

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dc.contributor.authorNasaruddin, Afnan Shazwan, author
dc.contributor.authorCharkowski, Amy O., advisor
dc.contributor.authorLeach, Jan E., committee member
dc.contributor.authorTrivedi, Pankaj, committee member
dc.contributor.authorHeuberger, Adam L., committee member
dc.date.accessioned2021-06-07T10:21:29Z
dc.date.available2022-06-02T10:21:29Z
dc.date.issued2021
dc.description.abstractSoft rot Pectobacteriaceae, Dickeya and Pectobacterium, are notorious for causing blackleg and soft rot diseases on more than 50% angiosperms such as potato, tomato, carrot, cabbage, and rice. In the United States, soft rot Pectobacteriaceae causes at least $40 million losses of potato each year. Flagellar motility is important for soft rot Pectobacteriaceae virulence. Chemotaxis, which controls flagellar motility towards a conducive environment or away from hostile conditions, is essential for initial stages of infection. Chemotaxis is mediated by chemoreceptors known as methyl-accepting chemotaxis proteins. Genomic analyses of soft rot Pectobacteriaceae and closely related animal pathogens and non-pathogenic bacteria in order Enterobacteriales showed that soft rot Pectobacteriaceae genomes are enriched in methyl-accepting chemotaxis proteins. Furthermore, soft rot Pectobacteriaceae methyl-accepting chemotaxis proteins contain more diverse ligand binding domains compared to other species in Enterobacteriales. This study suggests the importance of chemotaxis for soft rot Pectobacteriaceae pathogenicity and opens up possibilities for future research in targeting chemotaxis for plant disease management. In E. coli, the alternative sigma factor FliA is required for transcription initiation in motility and chemotaxis genes. To determine how chemotaxis is regulated in Dickeya, we conducted an RNA-sequencing experiment using a wild-type strain and a fliA mutant of D. dadantii 3937 grown in minimal media with glycerol or glucose. We found that the FliA sigma factor did not regulate methyl-accepting chemotaxis genes in Dickeya, several virulence genes were upregulated in glucose, and some genes postulated to be regulated by PecS were upregulated in glycerol. It is still a mystery as to which sigma factor regulates the chemotaxis genes in Dickeya, however, my work demonstrates that the regulation of chemotaxis in plant pathogens differs from closely related animal pathogens in the same order.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierNasaruddin_colostate_0053A_16561.pdf
dc.identifier.urihttps://hdl.handle.net/10217/232625
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectPectobacteriaceae
dc.subjectsoft rot disease
dc.subjectpotato
dc.subjectchemotaxis
dc.titleThe curious case of chemotaxis in soft rot Pectobacteriaceae
dc.typeText
dcterms.embargo.expires2022-06-02
dcterms.embargo.terms2022-06-02
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineAgricultural Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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