Repository logo
 

Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction

Simple item page
dc.contributor.authorRasmussen, Sara-Lesley, author
dc.contributor.authorSeidel, George E., Jr., advisor
dc.contributor.authorGraham, James K., committee member
dc.contributor.authorMcCue, Patrick M., committee member
dc.date.accessioned2007-01-03T05:16:21Z
dc.date.available2007-01-03T05:16:21Z
dc.date.issued2011
dc.description.abstractThis study compared the pregnancy rates between embryo transfer of bovine embryos produced in vitro with sexed vs control sperm and artificial insemination (AI) using sexed and unsexed sperm. Cleavage rates for oocytes fertilized with sexed vs control sperm were not different for two of the three bulls used, but were lower (p < 0.05) for the third bull sexed (44%) vs control sperm (70%). There were fewer transferable blastocysts produced per oocyte with sexed sperm (9-19%) than for unsexed sperm (18-26%); (p < 0.05). All cows were on an Ovsynch program to synchronize ovulation. Respective 60 d pregnancy rates at two Colorado dairies were as follows: control AI (43%, n=88; 43%, n=44); AI with X-sorted sperm (34%, n=82; 34%, n=62); embryo transfer (ET) with in vitro-produced (IVP) embryos using unsexed sperm (22%, n=68; 21%, n=39); and ET with IVP embryos using sexed sperm (7%, n=72; 37%, n=40). The pregnancy rate (day 60) for AI using sexed sperm was 78% of that of control sperm. ET pregnancy rates were generally lower than AI rates. At one dairy, abortions between days 32 and term were higher for X-sort ET pregnancies (79% n=14) than for AI control pregnancies (20% n=40); (P < 0.001). However, the other dairy experienced only a 12%, (n=17) abortion rate for transferred embryos produced from X-sorted sperm. The sex ratio of calves was similar to previous studies for AI with control sperm (52% bull calves, n=50), AI with X-sorted sperm (12% bull calves, n=40); ET with IVP embryos using unsexed sperm (50% bull calves, n=18); and ET with IVP embryos using sexed sperm (11% bull calves, n=18). Findings from this experiment indicate that embryo production with sexed sperm is not successful enough to be applied to large-scale dairies that already have successful breeding programs in place.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierRasmussen_colostate_0053N_10259.pdf
dc.identifier.urihttp://hdl.handle.net/10217/47443
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectassisted reproduction technologies
dc.subjectdairy cattle fertility
dc.subjectembryo transfer
dc.subjectIVF
dc.subjectpregnancy rates
dc.subjectsexed sperm
dc.titleApplying in vitro-produced embryos and sexed sperm to dairy cattle reproduction
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rasmussen_colostate_0053N_10259.pdf
Size:
620.05 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

2000-2019
Theses and Dissertations