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Chemotherapeutic responses in canine lymphoma models after treatment with the CHOP protocol

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dc.contributor.authorRamirez, Dominique, author
dc.contributor.authorWittenburg, Luke, author
dc.date.accessioned2016-04-27T13:37:52Z
dc.date.available2016-04-27T13:37:52Z
dc.date.issued2016
dc.descriptionIncludes bibliographical references.
dc.description.abstractIn both human and veterinary oncology, multi-drug resistance is a phenomenon where a cancer gains a cyto-protective effect against chemotherapeutics. Resistance is often witnessed when remitted cancers relapse and become untreatable. As an example, canine lymphomas are notorious for relapsing after treatment with the multi-drug CHOP protocol. While canonical drug efflux transporters have been implicated with the chemo-resistance phenotype, there are other transporters which might also contribute. Recent research has demonstrated that exposure to chemotherapeutics results in epigenetic changes to transporter gene expression; this could be a possible route for acquiring the resistance phenotype. What is still unknown, however, is a mechanistic understanding of the chemotherapy-transporter expression relationship. To address this void, we are focusing our research on three questions: 1) What are the temporal fluctuations in transporter expression following exposure to multi-drug regimens? 2) What patterns of epigenetic markers on transporter genes promote altered expression? 3) How does transporter expression correlate to protein levels in chemo-resistant lymphomas? We will address each of these questions using a panel of four chemo-sensitive canine lymphomas as our models, and the CHOP protocol as our drug regimen. We will expose the lymphomas to combinations of the CHOP protocol to mimic short- and long-term treatments, and monitor transporter expression via QT-PCR, and epigenetic changes via ChIP-assays. Additionally, protein levels will be monitored with LC-MS/MS methods to correlate expression with translation. We hypothesize that changes in transporter expression exhibit temporal and drug-dependent patterns.
dc.format.mediumborn digital
dc.format.mediumStudent works
dc.format.mediumposters
dc.identifier.urihttp://hdl.handle.net/10217/172423
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2016 Projects
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectchemoresistance
dc.subjectCHOP protocol
dc.subjectSLC transporters
dc.subjectSLC expression
dc.subjectcanine clinical lymphoma
dc.titleChemotherapeutic responses in canine lymphoma models after treatment with the CHOP protocol
dc.typeImage
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.disciplineClinical Sciences

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