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Effect of n-3 polyunsaturated fatty acid supplementation on circulating concentrations and mRNA expression of inflammatory cytokines in horses

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dc.contributor.authorSchauermann, Nicolette Lee, author
dc.contributor.authorHess, Tanja, advisor
dc.contributor.authorHan, Hyungchul, advisor
dc.contributor.authorHussey, Gisela, committee member
dc.contributor.authorEngle, Terry E., committee member
dc.date.accessioned2007-01-03T05:45:59Z
dc.date.available2007-01-03T05:45:59Z
dc.date.issued2010
dc.descriptionDepartment Head: William R. Wailes.
dc.description.abstractIntroduction: Studies in horses have shown an association between increased levels of the inflammatory cytokines and inflammatory conditions. Ingestion of n-3 polyunsaturated fatty acids (PUFA) can produce inflammatory mediators with the potential to down regulate the production of cytokines. Methods: Twenty one mares were blocked by age, body weight and body condition score and randomly assigned to one of three dietary treatment supplements. Treatments consisted of: 1) fish oil supplement and diet (FISH; Magnitude™); 2) flaxseed supplement and diet (FLAX; Nutra-Flax™); and 3) control diet (CON). Horses were supplemented for 12 weeks. Serum and white blood cells were collected on weeks 0, 4, 8 and 12 and assayed for TNFα protein concentrations and IL-1, IL-6, IL-10, and TNFα mRNA expression. Data was log transformed for normalization and analyzed by ANOVA. Significant differences (P<0.05) were compared by least square means analysis. Results: Statistical analysis of mRNA expression was not completed because of failure to produce consistent, reliable results from white blood cell samples. The serum analysis revealed no treatment effects, however FISH treated horses had a trend for lower TNFα than FLAX during week 4 (P=0.1202). Both the FISH and CON groups showed an increase between week 4 and 8 and a decrease between week 8 and 12 (P<0.035). The Flax group only showed a significant change between week 8 and 12 which was similar to the other treatments (P<0.040). Discussion: For more consistent results, a standard amount of whole blood should be used for mRNA expression analysis and include a RNA quality check procedure after RNA isolation. Results indicate that supplementation is not effective in altering TNFα concentrations in adult, healthy mares. The effect of n-3 PUFA may only be evident when inflammatory challenges are present as shown in previous studies of horses prone to laminitis, suffering from arthritis or stimulated with lipopolysaccharides.
dc.format.mediummasters theses
dc.identifier2010_Summer_Schauermann_Nicolette.pdf
dc.identifierETDF2010100003ANIS
dc.identifier.urihttp://hdl.handle.net/10217/39339
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleEffect of n-3 polyunsaturated fatty acid supplementation on circulating concentrations and mRNA expression of inflammatory cytokines in horses
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineAnimal Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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