Using a precision-cut lung slice co-culture paradigm to increase T-cell populations and model infection ex vivo
| dc.contributor.author | Ehrlich, Alexis T., author | |
| dc.contributor.author | Tobet, Stuart, advisor | |
| dc.contributor.author | Perera, Rushika, committee member | |
| dc.contributor.author | Magee, Christianne, committee member | |
| dc.date.accessioned | 2024-09-09T20:51:13Z | |
| dc.date.available | 2024-09-09T20:51:13Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Precision cut lung slices (PCLS) bridge a gap between in vivo and in vitro studies by maintaining anatomical organization with structural integrity and intercellular signaling pathways. Applications of PCLS have included the modeling of inflammatory lung diseases, metabolism studies, and drug development. In the lungs, immune responses are carried out by a network of T- and B- cells, the latter of which are resident. The limited resident T-cell population of the lung diminishes accurate representations of pathogen response capacity in PCLS. Addressing this, we set out to increase pulmonary T-cell populations ex vivo. We hypothesized that thymus and bone marrow-derived T-cells would work synergistically to populate the lung in co-culture experiments. A murine organotypic lung co-culture model was developed and characterized for tissue health and T-cell recruitment over 3 days ex vivo using adult neurobasal media with 4 mM glucose + 2% B27 supplement. Lung slices were cultured independently, with bone marrow, thymus, or both. Immune colonization of the lung was assessed using immunohistochemistry for CD3+ T-cells, CD19+ B-cells and ACK2+ cells. Cells were counted in alveolar and airway spaces after 3 days of culture. Our results demonstrate that lung co-cultured with thymus and bone significantly increased T-cell populations ex vivo, whereas lung co-cultured with thymus or bone alone did not significantly alter T-cell counts. Additionally, B-cells and C-Kit+ cell populations were not influenced by the culture paradigm. Using this paradigm, we went on to explore this lung co-culture paradigm when stimulated by an immune modulatory agent – LPS- and when an active lung infection is present using Pseudomonas aeruginosa. Lung and lung co-cultures had increases in T-cell counts after immune stimulation and infection. Additionally, the co-cultures further increased T-cell counts after treatment. More strikingly, the co-cultures influenced the degree of bacterial infection in the lung slices without altering the B-cell populations among cultures. Viral infections are also common pathogens that affect the lungs, so we examined then examined the ability of viral pathogens to infect precision cut lung slices. As we did not get an infection with live virus, we explored the effect of a viral mimic – Resiquimod- on lung co-cultures on the immune responses. Resiquimod and the co-cultures are both able to increase T-cell populations in lung slices ex vivo. These results suggest that the increased T-cell population corresponding with thymus and bone marrow co-culture could be a result of cell-cell interaction or the secretion of growth factors. Cell secretions or growth factor release could stimulate thymic secretion of T-cells or could stimulate T-cell proliferation in the lung, suggesting that co-culture with thymus and bone marrow can elicit a T-cell response ex vivo. T-cells are necessary for host-pathogen immune responses, most commonly by CD8+ T-cells but there are other populations of T-cells. Although there are limitations to the use of lung slices in infection studies, the results of the co-culture and increasing T-cells is a promising step to studying pathogen response capacity in the future ex vivo. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier | Ehrlich_colostate_0053N_18550.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/239171 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2020- | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.title | Using a precision-cut lung slice co-culture paradigm to increase T-cell populations and model infection ex vivo | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Biomedical Sciences | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
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