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Directed expression of R2R3 MYB transcription factors for ectopic suberin deposition

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dc.contributor.authorMcKay Whiteman, Angel, author
dc.contributor.authorMedford, June, advisor
dc.contributor.authorNishimura, Marc, committee member
dc.contributor.authorPeebles, Christie, committee member
dc.date.accessioned2023-01-21T01:24:03Z
dc.date.available2023-01-21T01:24:03Z
dc.date.issued2022
dc.description.abstractPlants are susceptible to many stresses, which can lead to reduced growth, decreased crop yield, or even plant death. Plants contain natural barriers to protect them from stresses, including the hydrophobic biopolymer suberin. Suberin protects plants against a variety of factors, including water loss, toxic ions, loss of essential nutrients, and entry of microorganisms. Plant roots contain a suberized barrier called the endodermis, which regulates the entry and exit of materials from the cortex to the vascular cylinder. While the endodermis protects the vascular cylinder, the cortex is left susceptible to stresses. Engineering a suberized barrier in the root epidermis could protect the cortex and provide an additional point of regulation. Multiple R2R3 type MYB transcription factors have been found to be involved in suberin biosynthesis. Expressing these transcription factors in the root epidermis could provide a suberin barrier for protection. I expressed five R2R3 MYB transcription factors in the root epidermis of Arabidopsis thaliana to determine whether their expression led to suberization of the root epidermis. Constitutive expression of one transcription factor, MYB84, led to increased epidermal suberin. Two homozygous lines were further analyzed and found to have decreased root growth. Gene expression results from one homozygous line suggest that MYB84 overexpression may lead to increased expression of suberin biosynthetic genes involved in synthesis of aliphatic suberin monomers and monomer transport. Further analysis of these transgenic plants could provide insight into the potential protective barrier the root epidermal suberin provides.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierMcKayWhiteman_colostate_0053N_17474.pdf
dc.identifier.urihttps://hdl.handle.net/10217/235941
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleDirected expression of R2R3 MYB transcription factors for ectopic suberin deposition
dc.typeText
dc.typeImage
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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