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Spn1, Spt4, Spt5, and Spt6 preserve chromatin structure over promoters and open reading frames

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dc.contributor.authorTonsager, Andrew Jordan, author
dc.contributor.authorStargell, Laurie A., advisor
dc.contributor.authorHansen, Jeffrey C., committee member
dc.contributor.authorSantangelo, Thomas, committee member
dc.contributor.authorArgueso, Juan Lucas, committee member
dc.date.accessioned2024-05-27T10:32:53Z
dc.date.available2025-05-20
dc.date.issued2024
dc.description.abstractThe eukaryotic chromatin landscape presents formidable nucleosomal barriers for processes that require access to DNA, such as transcription. These barriers are overcome through the action of many factors, including histone chaperones Spn1, Spt5, Spt6, and FACT and transcription elongation factor Spt4. However, it is poorly understood how each contributes to this process. To ascertain the role that these factors play on preserving chromatin structure over the genome, this thesis has utilized micrococcal nuclease digestion followed by sequencing (MNase-seq) to analyze chromatin protections in the yeast genome in cells expressing numerous mutant alleles of these factors. Extensive characterization of MNase-protected fragments in a wide range of sizes established that the essential histone chaperone Spn1 preserves both nucleosomal and subnucleosomal structures over both promoters and open reading frames across the genome. Additional analyses from existing MNase-seq datasets demonstrated the extent to which Spn1 and other RNAPII-associated factors maintain nucleosome features over genes of varied characteristics. The study of factors described in this thesis is performed in living cells, which have been genetically modified to express mutant alleles of chromatin factors. This thesis also describes a course-based undergraduate research experience (CURE) developed to introduce upper-level biochemistry students to techniques in yeast genome engineering in an authentic research setting.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierTonsager_colostate_0053A_18306.pdf
dc.identifier.urihttps://hdl.handle.net/10217/238508
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.rights.accessEmbargo expires: 05/20/2025.
dc.subjectcourse-based undergraduate research experience
dc.subjectyeast
dc.subjectSpn1
dc.subjectchromatin
dc.titleSpn1, Spt4, Spt5, and Spt6 preserve chromatin structure over promoters and open reading frames
dc.typeText
dcterms.embargo.expires2025-05-20
dcterms.embargo.terms2025-05-20
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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