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Pseudo Pelger-Huët anomalies as potential biomarkers for acute radiation dose in rhesus macaques (Macaca mulatta)

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dc.contributor.authorHayes, Joshua Michael, author
dc.contributor.authorJohnson, Thomas E., advisor
dc.contributor.authorBailey, Susan, committee member
dc.contributor.authorBrandl, Alexander, committee member
dc.contributor.authorWalrond, John, committee member
dc.date.accessioned2021-06-07T10:20:53Z
dc.date.available2021-06-07T10:20:53Z
dc.date.issued2021
dc.description.abstractThe potential for malicious use of radiation, or radiation accidents could potentially lead to acute, high radiation doses to members of the public. Following an acute accidental exposure to high doses of radiation, medical intervention is pivotal to the survivability of the patient and the sooner the appropriate measures are taken the better the odds for survival. Early estimates of acute accidental radiation doses can be determined via biomarkers such as dicentric chromosome analysis or scenario reconstruction using computer software. However, both take valuable time, and can be expensive. Here, potentially faster, and cheaper quantitative biomarkers for radiation exposure were evaluated in acutely exposed Rhesus Macaques from the Wake Forest School of Medicine, Department of Comparative Medicine. Increased frequencies of abnormal neutrophils in peripheral blood, referred to as pseudo Pelger-Huët anomalies (PPHAs), have been shown to be potential biomarkers of radiation exposure in several scenarios, including the 1958 Y-12 criticality accident and the radium dial painters. We have confirmed the PPHA morphology to be present in Rhesus Macaques and a dose response curve, a biokinetics model, and determination of background prevalence of the morphology has been constructed utilizing peripheral blood smears. The dose response curve consists of macaques that received doses ranging from 0 Gy to 8.5 Gy (LD90/30) and a blood smear at a common time point post-irradiation. The biokinetics model utilized only 4 Gy exposures and blood smears taken periodically over 3.1 years post-irradiation. Results show a linear correlation between PPHA concentration and acute radiation dose and the PPHA morphology appears stable over 3.1 years post-irradiation.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierHayes_colostate_0053A_16430.pdf
dc.identifier.urihttps://hdl.handle.net/10217/232563
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectdosimetry
dc.subjectpseudo Pelger-Huët anomaly
dc.subjectrhesus macaque
dc.subjectPelger-Huët anomaly
dc.subjectbiodosimetry
dc.subjectradiation biomarker
dc.titlePseudo Pelger-Huët anomalies as potential biomarkers for acute radiation dose in rhesus macaques (Macaca mulatta)
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineEnvironmental and Radiological Health Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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