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Characterization of the dissociation equilibria of the histone H3H4 tetramer using sedimentation velocity analytical ultracentrifugation

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dc.contributor.authorConnolly, Mark Edward, author
dc.contributor.authorHansen, Jeffrey, advisor
dc.contributor.authorNyborg, Jennifer, committee member
dc.contributor.authorWalrond, John, committee member
dc.date.accessioned2020-06-22T11:52:49Z
dc.date.available2020-06-22T11:52:49Z
dc.date.issued2020
dc.description.abstractBy performing sedimentation velocity analytical ultracentrifugation experiments under various ionic and pH conditions the kd can be measured. This system was found to be pH dependent with a change of Svedberg (S) value distribution in between pH 5.2 and pH 5.5. The H3H4 system was found to be an interacting one due to the change in S value distribution with increasing the histone protein concentration. We found that the S value distribution is highly dependent on the ionic conditions of the solution with 2M NaCl solution showing higher S values then 5mM KPO4 solution at the same concentration and pH. Oddly enough adding HEPES to a KPO4 buffer will destabilize the H3H4 species present with 5mM KPO4 having a higher S value distribution than 5mM KPO4 10mM HEPES at the same concentration and pH. I was unable to model these systems to a H3H4 dimer to tetramer equilibrium model which leads me to believe the conditions I was using did not stabilize the tetramer save for the 2M NaCl.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierConnolly_colostate_0053N_15993.pdf
dc.identifier.urihttps://hdl.handle.net/10217/208471
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectdimer
dc.subjectH3H4
dc.subjecttetramer
dc.subjectdissociation
dc.subjectAUC
dc.subjecthistone
dc.titleCharacterization of the dissociation equilibria of the histone H3H4 tetramer using sedimentation velocity analytical ultracentrifugation
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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