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Comparison of an antioxidant source and antioxidant plus BCAA on athletic performance and post exercise recovery of horses

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dc.contributor.authorKent, Emily, author
dc.contributor.authorHess, Tanja, advisor
dc.contributor.authorColeman, Stephen, committee member
dc.contributor.authorHamilton, Karyn, committee member
dc.contributor.authorBruemmer, Jason, committee member
dc.date.accessioned2022-05-30T10:21:37Z
dc.date.available2024-05-24T10:21:37Z
dc.date.issued2022
dc.description.abstractAntioxidant supplementation has been shown to decrease post-exercise oxidative stress but can lead to decreased post-exercise muscle protein synthesis. The objective of this study was to compare the effects of the supplementation with a control feed with low antioxidant content (CON) to a high antioxidant feed (AO), versus a high antioxidant and branched-chain amino acid feed (BCAO) on post-exercise protein synthesis and oxidative stress. Our hypothesis is that supplementing AO with BCAA will reduce oxidative stress without hindering muscle protein synthesis. Eighteen mixed breed conditioned polo horses were assigned to one of the three treatments. All horses consumed the CON diet for 30 days and were then assessed using a lactate threshold test (LT). One hour later and following subsequential LT, horses were assigned to the experimental groups and given their treatments. Follow-up LTs were conducted on days 15 and 30 of supplementation. Blood was collected before, two and four hours after LT, and oxidative stress was assessed by determining glutathione peroxidase, superoxide dismutase and malondialdehyde concentrations by ELISA. Muscle biopsies were taken before and 4 hours after LT and analyzed for the expression of protein synthesis by RT-PCR. Results were analyzed in a mixed model by ANOVA and compared by LSM. A reduction of oxidative stress was found over time (P<0.050) with no treatment effect (P>0.050) when using the measured oxidative stress parameters mentioned above. An upregulation in the production of mRNA transcripts related to muscle protein synthesis after exercise was found for muscle primers CD36, CPT1, PDK4, MyF5, and Myogenin (P<0.050). There was a treatment by exercise effect for MyoD (P=0.0041), where AO was upregulated the most after exercise compared to BCAO and CON. MRF4 had a time by treatment effect (p=0.045) where AO was upregulated from day 0 to day 15 and 30 compared to BCAO and CON. This study demonstrated post-exercise muscle synthesis with no advantage of AO plus BCAA compared to AO.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierKent_colostate_0053N_17179.pdf
dc.identifier.urihttps://hdl.handle.net/10217/235231
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.rights.accessEmbargo Expires: 05/24/2024
dc.subjectbranched-chain amino acids
dc.subjectoxidative stress
dc.subjectantioxidants
dc.subjectpolo horses
dc.subjectmuscle protein
dc.titleComparison of an antioxidant source and antioxidant plus BCAA on athletic performance and post exercise recovery of horses
dc.typeText
dcterms.embargo.expires2024-05-24
dcterms.embargo.terms2024-05-24
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineAnimal Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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