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The role of the human Translationally Controlled Tumor Protein (TCTP) in mitosis

dc.contributor.authorBuechler, Betsy Lauren, author
dc.contributor.authorDeluca, Jennifer G., author
dc.date.accessioned2007-01-03T04:38:57Z
dc.date.available2007-01-03T04:38:57Z
dc.date.issued2009
dc.description.abstractMitosis is the complex process that results in division of DNA and other cellular components into two daughter cells. Successful cell division requires that microtubules of the mitotic spindle attach to the kinetochores of mitotic chromosomes. These attachments are used for both aligning chromosomes at the spindle equator and for the physical separation of sister chromosomes during anaphase. In the study of how microtubules and kinetochores work together during mitosis, researchers are searching for new proteins that may help to piece together this complex puzzle. The Translationally Controlled Tumor Protein (TCTP) has been suggested to play a role in mitotic cell division, as it has been immuno-localized to the mitotic spindle, however, its precise function in mitosis remains unknown. The goal of my project is to determine the role of human TCTP in mitosis. Thus far, my research has shown that TCTP is located at the microtubules during mitosis, and when knocked down by siRNA treatment, human HeLa H2B-GFP cells (GFP-tagged chromosomes) are either unable to complete mitosis or take an extended time to do so. Currently, I am undergoing procedures to pick the most suitable human cell line in which to study the roles of TCTP during mitosis (HeLa-S3, HeLa-Kyoto, or HeLa H2B-GFP), and a mitotic index and characterization of this cell line. In the future, I plan to look in to the association between F-actin and TCTP, recently brought to light by Bazile et al, 2009.
dc.description.awardHonors by College.
dc.format.mediumStudent works
dc.format.mediumposters
dc.identifier.urihttp://hdl.handle.net/10217/30014
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2009 Projects
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectmicrotubule
dc.subjectkinetochore
dc.subjectmitotic cell division
dc.subjectTCTP
dc.subjecttranslationally controlled tumor protein
dc.subjectmitosis
dc.titleThe role of the human Translationally Controlled Tumor Protein (TCTP) in mitosis
dc.typeStillImage
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineNatural Sciences
thesis.degree.disciplineBiochemistry

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The role of the human Translationally Controlled Tumor Protein (TCTP) in mitosis

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