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Characterizing porous protein crystal materials for applications in nanomedicine and nanobiotechnology

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dc.contributor.authorHartje, Luke Fredrick, author
dc.contributor.authorSnow, Christopher D., advisor
dc.contributor.authorHo, P. Shing, committee member
dc.contributor.authorPeersen, Olve B., committee member
dc.contributor.authorMcCullagh, Martin, committee member
dc.date.accessioned2018-09-10T20:05:15Z
dc.date.available2020-09-06T20:04:15Z
dc.date.issued2018
dc.description.abstractProtein crystals are biologically derived, self-assembling, porous structures that have been used for decades in structure determination via X-ray diffraction. Recently, however, there has been increased interest in utilizing protein crystals for their unique material properties—most notably, their highly ordered porous structure, innate biocompatibility, and chemical plasticity. The diverse topologies of protein crystals and the relative ease with which their chemical properties can be altered via genetic mutation or chemical modification offers a wider and more dynamic design palette than existing chemically-synthesized nanoporous frameworks. These traits make protein crystals an attractive new material for applications in nanomedicine and nanobiotechnology. The intent of this project is to demonstrate the application potential of porous protein crystal materials for use in nanostructured devices. This work highlights our efforts to: experimentally and computationally investigate macromolecular transport and interaction energies within a large-pore protein crystal environment using time-lapse confocal microscopy, bulk equilibrium adsorption, and hindered diffusion simulation; assess the cytocompatibility of various cross-linking chemistries for the production of biostable protein crystal materials for use in biologically sensitive environments; and create multifunctional textiles by covalently attaching various cross-linked protein crystals to cellulose fibers in woven cotton fabrics. By pursuing this research, we hope to better understand porous protein crystal materials and leverage that knowledge to design advanced nanostructured devices for applications in medicine and biotechnology.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierHartje_colostate_0053A_15014.pdf
dc.identifier.urihttps://hdl.handle.net/10217/191422
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectbiomaterial
dc.subjectfunctionalized textiles
dc.subjectscaffold material
dc.subjectcytocompatibility
dc.subjectadsorption-coupled diffusion
dc.subjectnanoporous
dc.titleCharacterizing porous protein crystal materials for applications in nanomedicine and nanobiotechnology
dc.typeText
dcterms.embargo.expires2020-09-06
dcterms.embargo.terms2020-09-06
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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