Repository logo
 

Phytoalexin deficient4 (PAD4): a plant defense regulatory gene with distinct alternative splicing patterns in tomato (Solanum lycopersicum) and soybean (Glycine max)

Simple item page
dc.contributor.authorSchmidt, Rebecca, author
dc.contributor.authorNalam, Vamsi, advisor
dc.contributor.authorArgueso, Cristiana, committee member
dc.contributor.authorReddy, Anireddy, committee member
dc.date.accessioned2023-06-01T17:27:00Z
dc.date.available2023-06-01T17:27:00Z
dc.date.issued2023
dc.description.abstractAlternative splicing is an important post-transcriptional regulatory mechanism that contributes to a plant's ability to perceive and respond to a variety of biotic and abiotic stressors. Alternative splicing has a documented role in plant immunity, as many R genes, which are important for plant defense against specialized pathogens, undergo alternative splicing in response to pathogen perception. Despite this, the role of alternative splicing in other components of plant defense responses is not well documented. As transcriptome data diversify to include more species and conditions, the extent of alternative splicing in plants has become apparent. PHYTOALEXIN DEFICIENT4 (PAD4), plays an integral role in plant defense signaling to biotic stressors, and in regulating responses to abiotic stresses. PAD4 undergoes alternative splicing in Soybean (Glycine max). Additionally, the expression pattern of Glycine max PAD4, GmPAD4, and its splice variant GmPAD4-AS1 are further characterized in early growth stages. We hypothesize PAD4 produces full-length and alternatively spliced transcripts in multiple species, and that PAD4 gene structure may influence the occurrence of alternatively spliced transcripts. Here we characterize alternative splicing of PAD4 in tomato (Solanum lycopersicum), identifying two splice variants. We also investigate the conservation of PAD4 intron-exon structure conservation across diverse species. PAD4 expression patterns are characterized using available expression data.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierSchmidt_colostate_0053N_17609.pdf
dc.identifier.urihttps://hdl.handle.net/10217/236560
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectPAD4
dc.subjectphytoalexin deficient4
dc.subjecttomato
dc.subjectphytoalexin deficient 4
dc.subjectalternative splicing
dc.subjectsoybean
dc.titlePhytoalexin deficient4 (PAD4): a plant defense regulatory gene with distinct alternative splicing patterns in tomato (Solanum lycopersicum) and soybean (Glycine max)
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineAgricultural Biology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Schmidt_colostate_0053N_17609.pdf
Size:
1.91 MB
Format:
Adobe Portable Document Format
Download

Collections

2020-
Theses and Dissertations