Dataset associated with “An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale”

de Figueiredo, Marcelo R. A.; Küpper, Anita; Malone, Jenna M.; Petrovic, Tijana; de Figueiredo, Ana Beatriz T. B.; Campagnola, Grace; Peersen, Olve B.; Prasad, Kasavajhala V.S.K.; Patterson, Eric L.; Reddy, Anireddy S. N.; Kubeš, Martin F.; Napier, Richard; Dayan, Franck E.; Preston, Christopher; Gaines, Todd A.

Dataset associated with “An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale”

dc.contributor.author	de Figueiredo, Marcelo R. A.
dc.contributor.author	Küpper, Anita
dc.contributor.author	Malone, Jenna M.
dc.contributor.author	Petrovic, Tijana
dc.contributor.author	de Figueiredo, Ana Beatriz T. B.
dc.contributor.author	Campagnola, Grace
dc.contributor.author	Peersen, Olve B.
dc.contributor.author	Prasad, Kasavajhala V.S.K.
dc.contributor.author	Patterson, Eric L.
dc.contributor.author	Reddy, Anireddy S. N.
dc.contributor.author	Kubeš, Martin F.
dc.contributor.author	Napier, Richard
dc.contributor.author	Dayan, Franck E.
dc.contributor.author	Preston, Christopher
dc.contributor.author	Gaines, Todd A.
dc.coverage.spatial	Port Broughton, South Australia, Australia	en_US
dc.coverage.temporal	2014-2021	en_US
dc.date.accessioned	2021-10-28T15:11:34Z
dc.date.available	2021-10-28T15:11:34Z
dc.date.issued	2021
dc.description	Output files from protein docking analysis of the wild-type IAA2 protein and the mutant IAA2 version containing a nine amino acid deletion. These residues were used as docking parameters in HADDOCK 2.4 (https://wenmr.science.uu.nl/haddock2.4/). Docking calculations were performed under expert level, deactivating the DNA/RNA functions and activating the Surface Contact Restrains to enforce contact between the molecules. Degron residues KNNN of SoIAA2 were assigned semiflexible properties during docking whereas the shorter connection in SoIAA2Δ9 was not allowed to be flexible to preserve the structural integrity of PB1. The binding affinities of the SoIAA2/TIR1 and SoIAA2Δ9/TIR1 biological complexes were calculated using PRODIGY (https://bianca.science.uu.nl/prodigy/) for all top 4 poses from the best HADDOCK clusters. Plots are in .html format and protein models are in .pdb format. Files are in TGZ after gzip compression.	en_US
dc.description	Department of Biology
dc.description	Department of Biochemistry & Molecular Biology
dc.description	Department of Agricultural Biology
dc.description.abstract	The natural auxin indole-3-acetic acid (IAA) is a key regulator of many aspects of plant growth and development. Synthetic auxin herbicides such as 2,4-D mimic the effects of IAA by inducing strong auxinic signaling responses in plants. To determine the mechanism of 2,4-D resistance in a Sisymbrium orientale (Indian hedge mustard) weed population, we performed a transcriptome analysis of 2,4-D-resistant (R) and -susceptible (S) genotypes that revealed an in-frame 27-nucleotide deletion removing 9 amino acids in the degron tail (DT) of the auxin co-receptor Aux/IAA2 (SoIAA2). The deletion allele co-segregated with 2,4-D resistance in recombinant inbred lines. Further, this deletion was also detected in several 2,4-D resistant field populations of this species. Arabidopsis transgenic lines expressing the SoIAA2 mutant allele were resistant to 2,4-D and dicamba. The IAA2-DT deletion reduced binding to TIR1 in vitro with both natural and synthetic auxins, causing reduced association and increased dissociation rates. This novel mechanism of synthetic auxin herbicide resistance assigns a new in planta function to the DT region of this Aux/IAA co-receptor for its role in synthetic auxin binding kinetics and reveals a potential biotechnological approach to produce synthetic auxin resistant crops using gene editing.	en_US
dc.description.sponsorship	This work was supported in part by funding from Dow AgroSciences, by the Grains Research and Development Corporation – Australian Government, grant UA00158, the Brazilian governmental scholarship from the National Council for Scientific and Technological Development (CNPq - 207387/2014-1) to M.R.A.F., and by the EU MSCA-IF project CrysPINs (792329) in support of M.F.K. RN and MFK acknowledge equipment access at the Warwick Integrative Synthetic Biology Centre, which is funded by BB/M017982/1.	en_US
dc.format.medium	GZIP
dc.format.medium	HTML
dc.format.medium	PDB
dc.format.medium	PDF
dc.identifier.uri	https://hdl.handle.net/10217/234027
dc.identifier.uri	http://dx.doi.org/10.25675/10217/234027
dc.language	English	en_US
dc.language.iso	eng	en_US
dc.publisher	Colorado State University. Libraries	en_US
dc.relation.ispartof	Research Data
dc.relation.isreferencedby	Figueiredo, M., A. Küpper, J. Malone, T. Petrovic, A.B. Figueiredo, G. Campagnola, O. Peersen, K. Prasad, E. Patterson, A.S.N. Reddy, M. Kubeš, R. Napier, F. Dayan, C. Preston, and T. Gaines. 2022. An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale. Proceedings of the National Academy of Sciences USA, 119(9), e2105819119. https://doi.org/10.1073/pnas.2105819119	en_US
dc.rights.license	The material is open access and distributed under the terms and conditions of the Creative Commons Public Domain "No rights reserved" (https://creativecommons.org/share-your-work/public-domain/cc0/).
dc.rights.uri	https://creativecommons.org/share-your-work/public-domain/cc0/
dc.subject	2,4-D	en_US
dc.subject	dicamba	en_US
dc.subject	herbicide resistance	en_US
dc.subject	Aux/IAA2	en_US
dc.subject	TIR1/AFB	en_US
dc.subject	plant hormones	en_US
dc.subject	target-site resistance	en_US
dc.title	Dataset associated with “An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale”	en_US
dc.type	Dataset	en_US