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The role of cell-secreted vesicles in equine ovarian follicle development

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dc.contributor.authorda Silveira, Juliano Coelho, author
dc.contributor.authorBouma, Gerrit, advisor
dc.contributor.authorCarnevale, Elaine, advisor
dc.contributor.authorWinger, Quinton, committee member
dc.contributor.authorVeeramacheneni, DN Rao, committee member
dc.contributor.authorTjalkens, Ronald, committee member
dc.date.accessioned2007-01-03T05:54:10Z
dc.date.available2014-09-30T05:54:08Z
dc.date.issued2013
dc.description.abstractOvarian follicular development is a process responsible for generating a gamete and steroid hormones, which are important for reproduction and general health. Failure of intrafollicular cell communication is one of the causes behind infertility. Recently, cell-secreted vesicles (microvesicles and exosomes) were described as mediators of cell communication through the transfer of bioactive material such as protein, mRNA and miRNA. Cell-secreted vesicles are present in different body fluids. The overall hypothesis is that cell-secreted vesicles are present in ovarian follicular fluid and are involved in regulating TGF-β signaling members during follicular development. In order to test this hypothesis we utilized the mare as an animal model due to the well described follicular dynamics and the easy access to sufficient experimental material. Firstly, we described the presence of microvesicles and exosomes in ovarian follicular fluid from pre-ovulatory follicles. Further we demonstrated the presence of cell-secreted vesicles markers such as miRNA and proteins. We also demonstrated that microvesicles are taken up by granulosa cell in vitro and in vivo. Secondly, we demonstrated the role of exosomes mediating regulation of TGF-β signaling members during follicular development at mid-estrous and pre-ovulatory stages. Thirdly, we demonstrated association between relative levels of TGF-β signaling members and exosomal miRNAs during follicular development in young and old mares. The data indicates that cell-secreted vesicles play an important role mediating cell proliferation and differentiation through the regulation of TGF-β signaling members during follicular development.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierdaSilveira_colostate_0053A_11898.pdf
dc.identifier.urihttp://hdl.handle.net/10217/80192
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectexosomes
dc.subjectovarian follicle development
dc.subjectmiRNAs
dc.subjectmicrovesicles
dc.subjectequine
dc.titleThe role of cell-secreted vesicles in equine ovarian follicle development
dc.typeText
dcterms.embargo.expires2014-09-30
dcterms.embargo.terms2014-09-30
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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