The extraction and measurement of adenosine triphosphate from marine sediment
Date
1976-05
Authors
Paul, E. A., author
Bancroft, K., author
Wiebe, W. J., author
American Society of Limnology and Oceanography, publisher
Journal Title
Journal ISSN
Volume Title
Abstract
A technique has been developed, using boiling sodium bicarbonate buffer, to extract adenosine triphosphate (ATP) from marine sediments and has been tested on a variety of sediments, including those with high organic content, clay, and carbonate. Recovery of ATP, as measured by the addition of bacteria of known ATP content to sediment, varied from 64-100%. The technique also was as effective as the conventional Tris buffer for extraction of ATP from both pure cultures of bacteria grown in broth and natural seawater samples.
Description
Publisher version: http://www.jstor.org/stable/2835285.
Rights Access
Subject
extraction
ATP standards
sediment
ATP technique
adsorptive characteristics
biomass