Proteomic analysis of the effect of metabolic acidosis on the apical membrane of the renal proximal convoluted tubule
| dc.contributor.author | Walmsley, Scott J., author | |
| dc.contributor.author | Curthoys, Norman, advisor | |
| dc.contributor.author | Deluca, Jennifer, committee member | |
| dc.contributor.author | Dobos, Karen, committee member | |
| dc.contributor.author | Laybourne, Paul, committee member | |
| dc.contributor.author | Prenni, Jessica, committee member | |
| dc.date.accessioned | 2007-01-03T05:50:26Z | |
| dc.date.available | 2012-06-01T08:10:42Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | Metabolic acidosis is a physiological disturbance which results in a decrease in blood and extracellular pH and HCO3-. The renal response to this disturbance is initiated in the proximal convoluted tubule (PCT) of the kidney. At the PCT, the brush border membrane facilitates solute reabsorbtion and excretion of acid during acidosis. However, the extent of the global remodeling of proteins at the brush border remains mostly unknown. Therefore a proteomic investigation of the remodeling of theseproteins during metabolic acidosis at the brush border was completed. First, using LTQ mass spectrometry and spectral counting, an enrichment method was tested that analyzed brush border membrane vesicles (BBMV) from cortex versus those which were derived from purified proximal convoluted tubules. From these results we detected and hypothesized that enzymes of glucose metabolism localized at the brush border would be altered in abundance during acidosis at the PCT brush border. Next, we performed a quantitative analysis of the temporal response to metabolic acidosis during 1-d, 3-d and 7-d acidosis using Q-TOF mass spectrometry and spectral counting. As expected, the results indicated a decrease of enzymes of glucose metabolism including Fructose-1,6-bisphosphatase 1 and Enolase A. Aldolase A was found to be transiently decreased during 1-d and 3-d acidosis. In addition, the Na+-glucose transporter 2 was found to be transiently increased during 1-d and 3-d acidosis. Finally, to confirm these abundance changes detected using spectral counting, an accurate mass and time tag method was developed. Using this method, we successfully developed an AMT database of the previously identified spectra. This database was used to match peptides detected using QTOF-LC-MS to the previously identified peptides. Peptide abundance by spectral counting was validated using the more accurate peak intensities and were generally in concordance with those abundance measurements using spectral counting. The developed model suggested a mechanism for internalization of these enzymes of glucose metabolism in support of glutamine metabolism, which is central to the cellular response to acidosis by the PCT. | |
| dc.format.medium | born digital | |
| dc.format.medium | doctoral dissertations | |
| dc.identifier | Walmsley_colostate_0053A_10356.pdf | |
| dc.identifier.uri | http://hdl.handle.net/10217/52136 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2000-2019 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | renal physiology | |
| dc.subject | mass spectrometry | |
| dc.subject | proteomics | |
| dc.subject | metabolic acidosis | |
| dc.title | Proteomic analysis of the effect of metabolic acidosis on the apical membrane of the renal proximal convoluted tubule | |
| dc.type | Text | |
| dcterms.embargo.expires | 2012-06-01 | |
| dcterms.embargo.terms | 2012-06-01 | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Cell and Molecular Biology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy (Ph.D.) |
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