Molecular epidemiology of leprosy: an update

Avanzi, Charlotte, author; Singh, Pushpendra, author; Truman, Richard W, author; Suffys, Philip N, author

Molecular epidemiology of leprosy: an update

dc.contributor.author	Avanzi, Charlotte, author
dc.contributor.author	Singh, Pushpendra, author
dc.contributor.author	Truman, Richard W, author
dc.contributor.author	Suffys, Philip N, author
dc.date.accessioned	2022-01-28T12:56:14Z
dc.date.available	2022-01-28T12:56:14Z
dc.date.issued	2020-12
dc.description	This pre-print paper was published in Infection Genetic and evolution but is not open access (https://www.sciencedirect.com/science/article/pii/S1567134820304123?via%3Dihub#ks0005).	en_US
dc.description	ZIP file includes XLSX spreadsheet.
dc.description.abstract	Molecular epidemiology investigations are notoriously challenging in the leprosy field mainly because the inherent characteristics of the disease as well as its yet uncultivated causative agents, Mycobacterium leprae and M. lepromatosis. Despite significant developments in understanding the biology of leprosy bacilli through genomic approaches, the exact mechanisms of transmission is still unclear and the factors underlying pathological variation of the disease in different patients remain as major gaps in our knowledge about leprosy. Despite these difficulties, the last two decades have seen the development of genotyping procedures based on PCR-sequencing of target loci as well as by the genome-wide analysis of an increasing number of geographically diverse isolates of leprosy bacilli. This has provided a foundation for molecular epidemiology studies that are bringing a better understanding of strain evolution associated with ancient human migrations, and phylogeographical insights about the spread of disease globally. This review discusses the advantages and drawbacks of the main tools available for molecular epidemiological investigations of leprosy and summarizes various methods ranging from PCR-based genotyping to genome-typing techniques. We also describe their main applications in analyzing the short-range and long-range transmission of the disease. Finally, we summarise the current gaps and challenges that remain in the field of molecular epidemiology of leprosy.	en_US
dc.format.medium	born digital
dc.format.medium	articles
dc.identifier.bibliographicCitation	Charlotte Avanzi, Pushpendra Singh, Richard W. Truman, Philip N. Suffys, Molecular epidemiology of leprosy: An update, Infection, Genetics and Evolution, Volume 86, 2020, 104581, ISSN 1567-1348, https://doi.org/10.1016/j.meegid.2020.104581.
dc.identifier.doi	https://doi.org/10.1016/j.meegid.2020.104581
dc.identifier.uri	https://hdl.handle.net/10217/234333
dc.language	English
dc.language.iso	eng
dc.publisher	Colorado State University. Libraries
dc.relation.ispartof	Faculty Publications
dc.rights.license	This manuscript version is made available under and distributed under the terms and conditions of the Creative Commons CC BY-NC-ND: Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	leprosy	en_US
dc.subject	molecular epidemiology	en_US
dc.subject	transmission	en_US
dc.subject	phylogeny	en_US
dc.subject	non-human reservoir	en_US
dc.subject	drug resistance	en_US
dc.title	Molecular epidemiology of leprosy: an update	en_US
dc.type	Text