Repository logo

Relationships between plasma cytokines, leukocyte telomere length, serum lipid profile, and nutrient intake in healthy adults following a 4-week dietary intervention study

dc.contributor.authorHarbison, Gregory James, author
dc.contributor.authorRyan, Elizabeth P., advisor
dc.contributor.authorBailey, Susan M., committee member
dc.contributor.authorTjalkens, Ronald B., committee member
dc.contributor.authorWeir, Tiffany L., committee member
dc.description.abstractColorectal cancer is the third most commonly diagnosed cancer worldwide and the fourth leading cause of cancer-related death. The etiology of colorectal cancer is predominately attributed to modifiable lifestyle factors that promote chronic inflammation, and only 20% of colorectal cases are credited to hereditary syndromes. Specifically, recent nutritional studies have suggested that diet modification is a promising lifestyle intervention for reducing systemic inflammation and promoting colorectal cancer prevention and remission. In particular, rice and navy beans have been identified as two foods with anti-inflammatory and anti-neoplastic properties that warrant evaluation for chemoprevention through dietary supplementation in humans. In this study, plasma cytokines (IL-2, IL-4, IL-6, IL-8, IL-10, TNF, and VEGF) and leukocyte telomere length were measured at baseline, two weeks, and four weeks in individuals with and without a history of colorectal cancer who consumed a diet supplemented with rice bran, navy beans, or a placebo-control for 28 days. Serum lipid profile and nutrient intake were also measured. At baseline, the three diet intervention groups had no significant differences in cytokine concentration, telomere length, or lipid profile. At the end of the study, individuals with a history of colorectal cancer who consumed the navy bean supplemented diet had significantly higher plasma TNF and VEGF concentrations than individuals consuming the control diet. Otherwise, at the end of the study, no significant differences in cytokine concentration or telomere length between groups existed. Additionally, compared to males, females with a history of colorectal cancer had significantly longer telomeres at baseline but not at four weeks. Females with a history of colorectal cancer also had significantly lower IL-4, IL-6, and IL-10 at baseline, but no significant difference was found at four weeks. Linear correlation analysis on repeated measures that adjusted for sex, age, and total energy intake showed significant correlations between several study variables. Telomere length was inversely correlated with age, serum triglyceride level, carbohydrate intake, and saturated fat intake. IL-2 and IL-4 concentrations were inversely correlated with α-Tocopherol intake. IL-8 was inversely correlated with vitamin B3 intake. VEGF was positively correlated with vitamin B9 intake. Total serum cholesterol was positively correlated with saturated fat intake and inversely correlated with β-Carotene intake. Serum LDL was inversely correlated with β-Carotene intake, and serum HDL was positively correlated with intake of saturated fat and linolenic acid. Triglyceride level was inversely correlated with intake of β-Carotene and fiber and was positively correlated with selenium intake. Finally, comparison of two experimental methods for telomere length measurement showed positive but inconclusive correlations.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.publisherColorado State University. Libraries
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see
dc.titleRelationships between plasma cytokines, leukocyte telomere length, serum lipid profile, and nutrient intake in healthy adults following a 4-week dietary intervention study
dcterms.rights.dplaThis Item is protected by copyright and/or related rights ( You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). and Radiological Health Sciences State University of Science (M.S.)


Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
18.91 MB
Adobe Portable Document Format