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Interaction of erythrocytes (RBC's) with nanostructured surfaces

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dc.contributor.authorVirk, Harvinder Singh, author
dc.contributor.authorPopat, Ketul C., advisor
dc.contributor.authorGhosh, Soham, committee member
dc.contributor.authorLi, Vivian, committee member
dc.date.accessioned2022-08-29T10:16:21Z
dc.date.available2023-08-22T10:16:21Z
dc.date.issued2022
dc.description.abstractTitanium and its alloys are used to make different blood-contacting medical devices such as stents, artificial heart valves, and catheters for cardiovascular diseases due to their superior biocompatibility. Thrombus formation begins on the surface of these devices as soon as they encounter blood. This leads to the formation of blood clots, which obstructs the flow of blood that leads to severe complications. Recent advancements in nanoscale fabrication and superhydrophobic surface modification techniques have demonstrated that these surfaces have antiadhesive properties and the ability to reduce thrombosis. In this study, the interaction of erythrocytes and whole blood clotting kinetics on superhydrophobic titanium nanostructured surfaces was investigated. These surfaces were characterized for their wettability (contact angle), surface morphology and topography (scanning electron microscopy (SEM)), and crystallinity (glancing angled X-Ray diffraction (GAXRD)). Erythrocyte morphology on different surfaces was characterized using SEM and overall cell viability was demonstrated through fluorescence microscopy. The hemocompatibility of these surfaces was characterized using commercially available assays: thrombin generation assay --> thrombin generation, hemolytic assay --> hemolysis, and complement convertase assay --> complement activity. The results indicate that superhydrophobic titanium nanostructured surfaces had lower erythrocyte adhesion, less morphological changes in adhered cells, lower thrombin generation, lower complement activation, and were less cytotoxic compared to control surfaces. Thus, superhydrophobic titanium nanostructured surfaces may be a promising approach to prevent thrombosis for several blood-contacting medical devices.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierVirk_colostate_0053N_17397.pdf
dc.identifier.urihttps://hdl.handle.net/10217/235646
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2020-
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjecterythrocytes
dc.subjecthemolysis
dc.subjectthrombin generation
dc.subjecthemocompatibility
dc.subjectcomplement convertase activity
dc.subjectnanostructured surfaces
dc.titleInteraction of erythrocytes (RBC's) with nanostructured surfaces
dc.typeText
dcterms.embargo.expires2023-08-22
dcterms.embargo.terms2023-08-22
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMechanical Engineering
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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