Automated analysis of 15N and 14C in biological samples
| dc.contributor.author | Paul, Eldor A., author | |
| dc.contributor.author | Harris, D., author | |
| dc.contributor.author | Marcel Dekker, Inc., publisher | |
| dc.date.accessioned | 2007-01-03T07:14:32Z | |
| dc.date.available | 2007-01-03T07:14:32Z | |
| dc.date.issued | 1989 | |
| dc.description.abstract | An automated method for the simultaneous analysis of total N, total C, 15N and 14C in small plant and soil samples is described. A commercial C-N analyser - continuous flow isotope ratio mass spectrometer (ANCA-MS) has been extended to also measure CO2 and collect 14CO2 produced by sample combustion. Samples containing 20 - 200 μg N and up to 5 mg C can be analysed directly with no sample preparation other than drying and fine grinding. The precision of total elemental analysis is comparable to that by conventional methods. The average standard deviation of 15N analyses of plant material at natural abundance was ±1 ‰. This is accurate enough for all 15N studies except those using natural abundance and possibly long term studies of soil organic matter. Recovery of 14C in test samples was 100%. The instrument can be operated by graduate students under supervision and operating costs are primarily for sample cups, combustion catalyst and quartz tubes. | |
| dc.format.medium | born digital | |
| dc.format.medium | articles | |
| dc.identifier.bibliographicCitation | Harris, D. and E. A. Paul, Automated Analysis of 15N and 14C in Biological Samples. Communications in Soil Science and Plant Analysis 20, no. 9-10 (1989): 935-947. https://dx.doi.org/10.1080/00103628909368127. | |
| dc.identifier.doi | https://dx.doi.org/10.1080/00103628909368127 | |
| dc.identifier.uri | http://hdl.handle.net/10217/85707 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | Faculty Publications | |
| dc.rights | ©1989 Marcel Dekker, Inc.. | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | 14C | |
| dc.subject | biological transformation | |
| dc.subject | carbon | |
| dc.subject | tracers | |
| dc.subject | nitrogen | |
| dc.subject | 15N | |
| dc.title | Automated analysis of 15N and 14C in biological samples | |
| dc.type | Text |
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