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Clustering of non-conducting Kv2.1 channels induces endoplasmic reticulum/plasma membrane junctions and forms cell-surface trafficking hubs

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dc.contributor.authorFox, Philip Douglas, author
dc.contributor.authorTamkun, Michael M., advisor
dc.contributor.authorAmberg, Gregory C., committee member
dc.contributor.authorVigh, Jozsef, committee member
dc.contributor.authorTsunoda, Susan, committee member
dc.contributor.authorGarrity, Deborah M., committee member
dc.date.accessioned2007-01-03T05:57:17Z
dc.date.available2016-01-31T06:30:24Z
dc.date.issued2014
dc.description.abstractThe voltage-gated K+ channel, Kv2.1, is expressed widely in the mammalian CNS, where it carries the majority of the delayed-rectifier current. The Kv2.1 current facilitates high-frequency action potential firing by promoting the repolarization of the membrane potential and subsequent recovery of voltage-gated Na+ channels from inactivation. Furthermore, Kv2.1 displays a unique cell-surface localization to dense, micron-sized clusters which are sensitive to neuronal insults such as glutamate excitotoxicity. The following dissertation presents original research extending our knowledge of the Kv2.1 K+ channel. The majority of Kv2.1 channels are held in a non-conducting state which is incapable of fluxing K+ in response to membrane potential depolarization. These non-conducting channels tend to localize to the micron-sized clusters which distinguish Kv2.1. Non-conducting, clustered Kv2.1 channels remodel the cortical endoplasmic reticulum (cER) into tight connections with the plasma membrane (PM), likely through a direct interaction. Trafficking of membrane proteins, both exo- and endocytosis are localized to the perimeter of the Kv2.1-induced ER/PM contacts by virtue of remodeling the cER underneath the Kv2.1 clusters. Thus the clustering of Kv2.1 functions to bring protein trafficking and intermembrane signaling together at the neuronal soma.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierFox_colostate_0053A_12748.pdf
dc.identifier.urihttp://hdl.handle.net/10217/88429
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectER/PM junctions
dc.subjecttrafficking
dc.subjectpotassium channel
dc.subjectnon-conducting
dc.subjectKv2.1
dc.subjectendoplasmic reticulum
dc.titleClustering of non-conducting Kv2.1 channels induces endoplasmic reticulum/plasma membrane junctions and forms cell-surface trafficking hubs
dc.typeText
dcterms.embargo.expires2016-01-31
dcterms.embargo.terms2016-01-31
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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