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Cytokines, antibodies and plasma viremia of cats infected with feline immunodeficiency virus

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dc.contributor.authorWood, Britta Ann, author
dc.contributor.authorVandeWoude, Sue, advisor
dc.contributor.authorAvery, Paul, committee member
dc.contributor.authorZabel, Mark, committee member
dc.contributor.authorHussey, Gisela, committee member
dc.date.accessioned2007-01-03T04:54:56Z
dc.date.available2014-06-30T04:54:32Z
dc.date.issued2013
dc.description.abstractFeline immunodeficiency viruses (FIVs) are naturally occurring lentiviruses (family Retroviridae) of felid species, including domestic and wild cats. Studies on FIVs are beneficial for understanding the host immune response associated with disease progression (e.g., domestic cat FIV) or the viral kinetics and molecular ecology associated with naturally occurring infections in wildlife (e.g., bobcat and mountain lion FIVs). Here we describe the development and validation of the following microsphere immunoassays (MIAs) for evaluating the cytokine and antibody response of domestic cats: i) the quantification of cytokines (interferon gamma (IFNγ), interleukin (IL)-10, and IL-12/IL-23) in cell culture supernatant, and ii) the quantification of these cytokines in plasma; iii) the quantification of total IgG and IgA in plasma, and iv) the detection of IgG and IgA antibodies to feline CD134 (the primary cell receptor for FIV), and FIV capsid (CA) and surface (SU) proteins in plasma. These assays were used to evaluate temporal cytokine and antibody responses of domestic cats experimentally infected with various FIV strains. To analyze viral RNA loads associated with naturally occurring FIV infections in bobcats or mountain lions, we are adapting existing quantitative PCR assays for use with plasma samples. The eight assays described here are/will be beneficial for addressing questions related to lentiviral immune response and viral kinetics.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierWood_colostate_0053A_11698.pdf
dc.identifier.urihttp://hdl.handle.net/10217/78877
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectmicrosphere immunoassays
dc.subjectplasma viremia
dc.subjectantibodies
dc.subjectcytokines
dc.subjectFIV/PLV
dc.titleCytokines, antibodies and plasma viremia of cats infected with feline immunodeficiency virus
dc.typeText
dcterms.embargo.expires2014-06-30
dcterms.embargo.terms2014-06-30
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMicrobiology, Immunology, and Pathology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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